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相关概念视频

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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相关实验视频

Updated: Jul 11, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

两个链的故事:通过链特定的测序来解码染色质复制.

Zhiming Li1, Zhiguo Zhang2

  • 1Institute for Cancer Genetics and Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA; West China School of Public Health and West China Fourth Hospital, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China.

Molecular cell
|January 17, 2025
PubMed
概括
此摘要是机器生成的。

像eSPAN和OK-seq这样的链特异性测序方法对于在复制过程中区分DNA链至关重要. 这些工具揭示了对DNA复制,基因素转移和真核生物中的表观遗传的洞察力.

关键词:
修复DNA损伤的修复复制DNA复制DNA复制DNA复制表观遗传遗传是一种表观遗传遗传.基因组维护是基因组的维护.核细胞组合组合的核细胞组合.父母基因组织蛋白转移.链特异性测序是指链特异性的测序.

更多相关视频

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome
05:22

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

相关实验视频

Last Updated: Jul 11, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome
05:22

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • DNA复制涉及不同DNA聚合酶 (Pol ε和Pol δ) 的带头和滞后链的不对称合成.
  • 在体内区分新生的DNA链对于理解复制动态和表观遗传是必不可少的.

研究的目的:

  • 审查特定链的测序方法的基本原则.
  • 要总结从应用这些技术在真核细胞中获得的机械洞察力.
  • 讨论当前染色体复制研究的局限性和未来方向.

主要方法:

  • 对特定链的测序策略的审查,包括蛋白质相关新生DNA (eSPAN) 的丰富和测序和奥卡扎基片段测序 (OK-seq).
  • 分析这些方法生成的数据,以研究DNA复制和表观遗传现象.

主要成果:

  • 应用eSPAN和OK-seq为DNA复制机制提供了关键的见解.
  • 这些技术已经揭示了诸如亲生组织激素转移和表观遗传等过程.
  • 综述综合了使用这些测序策略的各种研究的发现.

结论:

  • 链特异性测序对于剖析真核细胞DNA复制及其表观遗传后果是不可或缺的.
  • 目前的技术有局限性,需要进一步的技术创新.
  • 需要先进的方法来充分理解染色体复制的调节和动态.