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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jun 1, 2025

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
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通过使用原生RNA纳米孔测序来绘制RNA生物型的表观转录学景观.

Gregor Diensthuber1, Eva Maria Novoa2

  • 1Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain; Universitat Pompeu Fabra, Barcelona 08003, Spain.

Molecular cell
|January 17, 2025
PubMed
概括

直接RNA纳米孔测序 (DRS) 彻底改变了RNA修饰的研究,使不同类型的同时识别在单分子分辨率上成为可能. 这项技术提供了一种强大的方法来了解各种RNA生物型的表观转录学景观.

关键词:
基因组RNA的修改 基因组RNA的改变它们是mRNARNA.纳米孔测序的测序rRNARNArRNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNARNA这是一个tRNARNA.

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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

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相关实验视频

Last Updated: Jun 1, 2025

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物化学 生物化学

背景情况:

  • RNA修饰对于基因表达和细胞反应至关重要.
  • 研究RNA修饰的多样化和动态性质提出了重大挑战.
  • 直接RNA纳米孔测序 (DRS) 为全面的表体转录分析提供了一种新的解决方案.

研究的目的:

  • 审查DRS在表皮转录组研究中的开创性应用.
  • 突出DRS在研究各种RNA生物型中的应用.
  • 讨论DRS在RNA修饰研究中的未来潜力和挑战.

主要方法:

  • 直接RNA纳米孔测序 (DRS) 用于同时识别RNA修饰.
  • 为不同的RNA生物型量身定制的专门图书馆准备协议.
  • 用于检测自然和合成RNA修饰的生物信息工作流.

主要成果:

  • DRS可实现RNA修饰的单分子和单核酸解析.
  • 该技术适用于各种RNA生物型.
  • DRS有助于检测内源性和人工RNA修改.

结论:

  • DRS是一种用于全面表皮转录基因分析的变革性技术.
  • DRS化学的进步正在提高测序精度和产量.
  • 未来的研究将利用DRS来克服目前的局限性,并探索RNA修饰生物学中的新机遇.