哺乳动物mRNA中的大多数m5C修饰是非适应性的
Zheng Li1, Kai Mi1, Chuan Xu1,2
1Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China.
Molecular biology and evolution
|January 17, 2025
概括
哺乳动物中的大多数信使RNA5甲基化 (m5C) 修饰似乎不适应. 对人类和小鼠数据的分析表明,这些RNA修饰很少得到保存,并且可能有害,质疑它们广泛的生物学意义.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 进化生物学 进化生物学
背景情况:
- 使者RNA (mRNA) 5甲基化 (m5C) 是一种在真核生物中常见的内部RNA修饰.
- 虽然已知m5C会影响某些生物过程,但其在mRNA中的整体功能在很大程度上仍未被描述.
- 了解m5C的进化保护和适应意义对于其生物学解释至关重要.
研究的目的:
- 研究人类和小鼠蛋白质编码基因中m5C修饰的全基因组进化特征.
- 确定大多数mRNA m5C修饰是否具有功能性和进化性保存.
- 评估哺乳动物mRNA中m5C修饰的潜在适应价值.
主要方法:
- 分析了来自13个人类和13个小鼠组织的RNA测序数据.
- 在整个物种中对m5C修饰发生,分布和进化保护的全基因组评估.
- 对观察到的m5C修饰的潜在有害影响的估计.
主要成果:
- 在mRNA上发生m5C修饰的发生率在各种组织和物种中极其低.
- 在人类和小鼠之间,m5C的修饰在很大程度上是不共享的,并且缺乏显著的进化保护.
- 观察到的mRNA m5C修饰的很大一部分可能是有害的,这表明缺乏适应性功能.
结论:
- 哺乳动物mRNA中的大多数m5C修饰可能是不适应性的.
- 这些发现挑战了m5C和其他mRNA修饰的广泛生物学意义.
- 需要进一步的研究,以阐明少数保留或功能性的m5C网站的具体作用,如果有的话.
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