相关实验视频
Updated: Jun 1, 2025

09:50
Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures
Published on: June 28, 2017
8.7K
在冷保护剂加载期间消除透应激:对溶解物-溶剂运输的数学研究
Joseph R Kangas1, Christopher J Hogan1, John C Bischof1
1Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, United States of America.
Cryobiology
|January 17, 2025
概括
新的模型通过保持恒定的细胞体积,在冷保护剂加载 (CPA加载) 期间消除了透应激. 这种方法最大限度地减少了细胞损伤和死亡,提供了一种更安全,更强大的冷保存方法.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 低温生物学 低温生物学
背景情况:
- 冷保护剂的加载会引起透应激,导致细胞损伤和死亡.
- 目前的方法缺乏以模型为指导的策略来缓解这些透梯度.
- 在冷保护剂加载期间减少生命力损失对于有效的冷保存至关重要.
研究的目的:
- 开发一种以模型为导向的冷保护剂加载协议,以最大限度地减少透应激.
- 为了获得恒定细胞体积冷保护剂加载的分析解决方案.
- 为现有的CPA加载方法提供更安全,更强大的替代方案.
主要方法:
- 在恒定的细胞体积约束下对冷保护剂加载进行了两参数形式主义的改革.
- 衍生分析溶液用于短暂的细胞外透性和非透性冷保护剂度.
- 开发了使用细胞液压导电性 (Lp),膜透性 (Ps) 和细胞体积 (Vo) 的坡道和阶段性加载协议的近似值.
主要成果:
- 通过消除透应力,实现了无体积损失的冷保护剂负载.
- 提供了通过控制细胞外冷保护剂度来最大限度地减少透应激的协议.
- 证明拟议的协议在对参数不确定性和生物变异性方面具有稳定性.
- 表明没有体积损失的CPA加载发生在与传统方法相似的时间尺度上.
结论:
- 开发的分析解决方案和加载协议有效地消除了冷保护剂加载期间的透应激.
- 与传统策略相比,这种无体积损失的方法提供了一种更安全,更稳定的冷保存方法.
- 这些发现表明,在冷保护剂加载过程中改善细胞活力方面取得了重大进展.
相关概念视频
Osmosis and Osmotic Pressure of Solutions
39.0K
A number of natural and synthetic materials exhibit selective permeation, meaning that only molecules or ions of a certain size, shape, polarity, charge, and so forth, are capable of passing through (permeating) the material. Biological cell membranes provide elegant examples of selective permeation in nature, while dialysis tubing used to remove metabolic wastes from blood is a more simplistic technological example. Regardless of how they may be fabricated, these materials are generally...
39.0K
Recrystallization: Solid–Solution Equilibria
1.0K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.0K
Freezing Point Depression and Boiling Point Elevation
34.1K
Boiling Point Elevation
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
34.1K
Solution Equilibrium and Saturation
18.4K
Imagine adding a small amount of sugar to a glass of water, stirring until all the sugar has dissolved, and then adding a bit more. You can repeat this process until the sugar concentration of the solution reaches its natural limit, a limit determined primarily by the relative strengths of the solute-solute, solute-solvent, and solvent-solvent attractive forces. You can be certain that you have reached this limit because, no matter how long you stir the solution, undissolved sugar remains. The...
18.4K
Solution Formation
31.2K
There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride.
This selective...
This selective...
31.2K
Colloidal precipitates
500
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
500

