相关实验视频

Updated: Aug 14, 2026

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Published on: May 27, 2011

构建一个表达的人类KREMEN1的Vero细胞系,用于开发CVA6疫苗

Dongqing Zhang^1,2, Yuxiang Zou^1,2, Jiaying Wu^1,2

¹State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, 361102, PR China.

Virology journal

|January 17, 2025

概括

一个稳定过度表达KREMEN1 (KRM1) 的新的Vero细胞系有效地支持Coxsackievirus A6 (CVA6) 复制. 这一进展有助于开发用于手足口病 (HFMD) 的CVA6疫苗.

科学领域:

病毒学病毒学
疫苗学疫苗学疫苗学
细胞生物学细胞生物学

背景情况:

考克萨基病毒A6 (CVA6) 是全球手足口病 (HFMD) 爆发的重要原因之一.
目前缺乏CVA6疫苗对疾病控制构成了挑战.
维罗细胞被广泛用于疫苗生产,但很少支持CVA6的传播.

研究的目的:

开发一种强大的细胞培养系统,用于CVA6的传播.
为增强CVA6复制而设计Vero细胞.
评估工程细胞在CVA6疫苗开发中的潜力.

主要方法:

使用晶状病毒系统构建稳定过度表达KREMEN1 (KRM1) 的Vero细胞系.
在工程Vero-KRM1_#11细胞系中评估CVA6的传播效率.
在工程细胞中产生的CVA6的免疫性和保护功效的评估.

主要成果:

维罗-KRM1_#11细胞系有效地支持了CVA6感染和传播.
在Vero-KRM1_#11细胞中传播的CVA6达到与RD细胞 (约. 10^7 TCID50/mL).) 的情况.
纯化的CVA6诱导了类似的IgG和中和抗体反应,而抗血清剂在小鼠中提供了100%的保护.

结论:

表达KRM1的Vero细胞为CVA6传播提供了一个可行的平台.
工程细胞促进了用于疫苗开发的CVA6的产生.
这种方法有可能创造CVA6或多价HFMD疫苗.

关键词:

考克萨基病毒A6 人类的KREMEN1受体疫苗疫苗疫苗疫苗维罗细胞系的细胞系

更多相关视频

Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells

Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells

Published on: August 1, 2013

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

相关概念视频

Cell-mediated Immune Responses

Cell-mediated Immune Responses

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

Cytomegalovirus Disease

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...