在针对ON双极细胞后,视觉代码的增强恢复与光遗传疗法治疗视网膜质细胞相比
Jessica Rodgers1, Steven Hughes2, Aghileh S Ebrahimi1
1Faculty of Biology, Medicine & Health, University of Manchester, Manchester M13 9PT, UK.
概括
视力恢复的光遗传疗法显示出有前途. 针对ON双极细胞 (ON BCs) 进行素表达,与准视网膜质细胞 (RGCs) 相比,产生更丰富的视觉代码.
科学领域:
- 神经科学是一个神经科学.
- 眼科医生 眼科 眼科
- 生物医学工程 生物医学工程
背景情况:
- 光遗传疗法为光受体退化中的视力恢复提供了潜在的途径.
- 选择素表达的细胞类型对于恢复视力的质量至关重要.
研究的目的:
- 在ON双极细胞 (ON BCs) 和视网膜质细胞 (RGCs) 中进行视觉反应后的视觉反应的临床前比较.
- 评估针对不同视网膜细胞类型对恢复视觉信号的真实性和特征的影响.
主要方法:
- 利用在表达 Grm6- (ON BCs) 或 Brn3c- (RGCs) 细胞中表达素ReaChR 的视网膜变性小鼠.
- 视网膜和背侧生殖细胞核中单个单元分辨率的比较刺激-反应特征.
- 在视觉完整的视网膜中记录ReaChR驱动的反应,以评估视觉特征选择性.
主要成果:
- 无论是ONBC还是RGC的准都恢复了视觉反应,在不同的光线条件下具有相当的灵敏度和对比度编码能力.
- 针对RGC的结果是不那么可复制和更刻板的反应,极性,对比度灵敏度和时间调的多样性减少.
- 发现RGC向的素表达扰乱了正常视觉视网膜中个别RGC的视觉特征选择性.
结论:
- 虽然ON BC和RGC准策略都有效地恢复视觉反应,但ON BC准生成了更细致和多样化的视觉代码.
- 在光遗传视力恢复中,ON双极细胞准似乎更优越,可以实现更接近野生类型小鼠视觉代码.
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