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乳球菌乳酸菌亚种. 乳糖增强了Caenorhabditis elegans中的抗压力和宿主防御机制

Mohammad Shaokat Ali^1,2, Shamima Ahmed^2,3, Yoshihiko Tanimoto^3,4

¹Graduate School of Human Life Science, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi-Ku, Osaka 558-8585, Japan.

Journal of applied microbiology

|January 18, 2025

概括

益生菌 Lactococcus lactis 菌株 LL100933 和 LL12007 增强了 Caenorhabditis elegans 对病原体的生存率. 这些益生菌激活宿主防御通路,包括p38 MAPK和DAF-16,提高免疫力.

科学领域:

微生物学和免疫学微生物学和免疫学
遗传学和分子生物学遗传学和分子生物学
遗体学遗体学是一个学科.

背景情况:

益生菌通过调节宿主免疫反应来提供潜在的健康益处.
凯诺哈比迪斯 (Caenorhabditis elegans) 作为研究宿主-病原体相互作用和先天免疫力的模型生物.

研究的目的:

为了评估Lactococcus lactis亚种的影响. 乳腺菌株LL100933和LL12007对Caenorhabditis elegans宿主防御机制的研究.
阐明参与益生菌介导的对病原体和压力因素的保护的分子途径.

主要方法:

凯诺拉布狄氏菌被用大肠杆菌OP50养,并补充了L. lactis菌株LL100933或LL12007.
在病原性条件下 (沙门氏菌) 进行了生存测定.
使用RNA测序和基因本体学进行基因表达分析,对治疗和控制进行了分析.

主要成果:

与对照群相比,用L. lactis菌株LL100933或LL12007养C. elegans显著增加了对病原体的生存率.
益生菌诱导的保护依赖于DAF-16和pmk-1通路.
观察到免疫相关基因的升调,包括谷氨S转移酶,UDP-glucuronyl转移酶和C型莱克.

结论:

乳球菌乳酸菌亚种. 乳糖菌株LL100933和LL12007显示出作为增强宿主免疫力的益生菌的潜力.
这些发现强调了特定的L. lactis菌株在通过保存的信号通路来加强先天免疫防御中的作用.

关键词:

凯诺哈比迪斯的优雅的植物.乳球球菌 (lactococcus lactis) 是一种乳球菌.主机防御的主机防御抗压力抗压力抗压力

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