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隔离,净化和表征Lentinus edodes的多糖与亚临界水提取的多糖,增强了深度欧性溶剂

Chaoting Wen¹, Zhiqiang Ye¹, Guoyan Liu¹

¹College of Food Science and Engineering, Yangzhou University, Yangzhou, China.

Chemistry & biodiversity

|January 18, 2025

概括

这项研究开发了一种新的方法,使用加深欧性溶剂增强的亚临界水提取来分离强效的Lentinus edodes多糖化物 (LEP1和LEP2). 这些化合物表现出显著的抗氧化和免疫调节活性,LEP2显示出卓越的疗效.

科学领域:

生物化学生化学
食品科学食品科学食品科学
药理学药理学是指药理学的学科.

背景情况:

伦丁多糖 (Lentinus edodes polysaccharides,简称LEP) 以其对健康的益处而闻名.
生物活性多糖的有效提取和表征对于其应用至关重要.
亚临界水提取 (SWE) 和深溶解剂 (DES) 提供有前途的绿色提取技术.

研究的目的:

开发和优化一个用深度环氧溶剂 (DES) 增强的亚临界水提取 (SWE) 方法,用于提取Lentinus edodes多糖 (LEP).
为了净化和表征提取的多糖类 (LEP1和LEP2).
评估净化LEP分量的抗氧化和免疫调节活性.

主要方法:

使用一种新型的SWE提取LEP,并用DES方法增强.
使用 DEAE-52 纤维素和 Sephadex G-100 染色学来净化 LEP.
结构分析 (组成,分子量,链接分析) 和体外测定抗氧化剂 (DPPH,Fe2+化,ROS,MDA,CAT,SOD在HepG2细胞中) 和免疫调节 (细胞,NO,IL-6,IL-1β,TNF-α在RAW264.7细胞中) 活动.

主要成果:

两种纯化的多糖,LEP1和LEP2,分别具有不同的分子量和糖成分 (糖,葡萄糖,银糖).
无论LEP1和LEP2都表现出显著的自由基清除,金属化和活性氧物减少活动,以及调节HepG2细胞中的抗氧化酶.
LEP1和LEP2通过增强RAW264.7细胞的细胞容量,氧化生产和促炎细胞因子释放,表现出强大的免疫刺激作用,而LEP2表现出更强的活性.

结论:

用DES方法增强的SWE有效地从Lentinus edodes中提取生物活性多糖.
纯化的LEP分量 (LEP1和LEP2) 具有显著的抗氧化和免疫调节特性.
这项研究强调了LEP作为功能成分的潜力,以及开发的提取技术对其他多糖的有用性.

关键词:

抗氧化剂活性抗氧化剂活性提取提取提取提取高压热水高压热水免疫刺激活动的免疫刺激活动.结构上的阐明.

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