相关实验视频

Updated: Jun 1, 2025

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

在抗PD-1治疗的TAP1缺陷黑色素瘤中,PMN-MDSCs负责免疫抑制

Xiao Zhang¹, Kaijun Sun², Bingzheng Zhong¹

¹Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510013, Guangdong, China.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

|January 18, 2025

概括

准多态核核髓衍生的抑制细胞 (PMN-MDSCs) 可以逆转TAP1缺陷瘤中的免疫抑制. 消除PMN-MDSC增强了抗瘤免疫力,在免疫治疗期间为这些癌症提供了潜在的治疗策略.

科学领域:

免疫学免疫学免疫学
癌症生物学癌症生物学
分子生物学分子生物学

背景情况:

与抗原处理 (TAP) 相关的载体对于HLA I抗原呈现至关重要.
低调TAP1与瘤进展和瘤微环境中的髓质衍生抑制细胞 (MDSC) 的增加有关.
尚不完全了解MDSCs对TAP1缺乏瘤免疫细胞耗尽的影响.

研究的目的:

研究MDSCs在TAP1缺陷瘤的免疫微环境中的作用.
确定是否准PMN-MDSCs可以改变免疫抑制并增强抗瘤免疫力.
评估PMN-MDSC枯竭作为TAP1缺乏瘤在免疫检查点阻塞 (ICB) 治疗中的治疗策略的潜力.

主要方法:

已建立的TAP1缺陷黑色素瘤的小鼠模型.
使用抗Ly6G抗体来耗尽多态核核髓衍生抑制细胞 (PMN-MDSCs).
通过流细胞计,定量PCR和免疫组织化学分析了免疫细胞透,细胞因子水平 (TNF-α,IL-6,TGF-β) 和免疫细胞功能.

主要成果:

TAP1缺乏与增加的Ly6G+细胞透相关.
减少PMN-MDSC逆转了免疫抑制,增加了CD8+ T细胞和CD8+/Treg比率,但没有影响先天免疫细胞.
衰竭导致TNF-α和IL-6的升高,TGF-β的降低,表明活性抗瘤免疫反应.

结论:

在TAP1淘汰赛瘤中,PMN-MDSCs对于建立瘤原生免疫微环境至关重要.
针对PMN-MDSCs是TAP1缺乏瘤的有希望的治疗方法,特别是在ICB治疗期间.

关键词:

这就是PMN-MDSC.在TAP1中,它是TAP1.瘤微环境是一个微环境.

更多相关视频

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Published on: February 5, 2020

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.