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Rad5和Ubc4直接在Lys164的PCNA中在体外无处可见

Yixiong Hu¹, Kaiyang Liu¹, Xue Bai¹

¹Department of Biochemistry and Molecular Biology, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University, Tianjin, PR China.

The Journal of biological chemistry

|January 18, 2025

概括

发芽酵母蛋白Rad5直接无处不在增殖细胞核抗原 (PCNA),对DNA复制应激反应至关重要. 这种新的机制涉及多个无处不在的部位,包括Lys164,增强无错误的DNA修复.

科学领域:

分子生物学分子生物学
复制DNA复制DNA复制DNA复制
乌比基的信号传递

背景情况:

增殖细胞核抗原 (PCNA) 无处不在对于DNA复制应激反应至关重要.
已知Rad5,Ubc13-Mms2和Rad6-Rad18复合体可以无处不在地化PCNA.
以前的研究表明,Rad5在Okazaki碎片结合缺陷期间与Ubc4合作PCNA泛化,但缺乏生物化学证据.

研究的目的:

生物化学复制和表征PCNA的Rad5-Ubc4介导的无化.
阐明这种反应中涉及的机制和特定的泛化部位.

主要方法:

使用纯化的Rad5和Ubc4.4进行PCNA泛化反应的体外复制.
对PCNA无化产品和部位的生物化学分析.

主要成果:

Rad5 和 Ubc4 直接催化了 PCNA 的无化.
这种反应需要Rad5的HIRAN和RING域的协调功能.
PCNA在多个位点处于无处不在,其中Lys164是主要的目标.

结论:

在涉及Ubc4.4的反应中,Rad5直接在PCNA中无处不在.
这提供了生物化学证据,证明Rad5在复制应激过程中PCNA无化中的作用.
Rad5可能使用一种新的机制来实现无错误的DNA损伤耐受性,通过直接在Lys164.4处无处不在PCNA.

关键词:

对DNA损伤的反应反应这是一个PCNA,PCNA,PCNA.Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 Rad5 在UBC4中,UBC4就是UBC4.无处不在的合酶乌比奎丁-结合酶酶的结合无处不在的化

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