相关实验视频

Updated: Jun 1, 2025

Intra-lymph Node Injection of Biodegradable Polymer Particles

Intra-lymph Node Injection of Biodegradable Polymer Particles

Published on: January 2, 2014

可注射水凝辅助局部脂聚糖的输送改善了免疫检查点阻塞疗法

Jiaxuan Li¹, Kuirong Mao¹, Xiandi Meng¹

¹Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Institute of Immunology, The First Hospital, Jilin University, Changchun, Jilin, PR China; National-local Joint Engineering Laboratory of Animal Models for Human Diseases, Changchun, Jilin, PR China.

Acta biomaterialia

|January 18, 2025

概括

一种新型的热敏水凝可局部提供脂多糖 (LPS) 来重编程瘤相关巨细胞 (TAM),增强免疫检查点阻塞 (ICB) 疗法. 这种有针对性的方法改善了抗瘤免疫力和生存率,没有显著的毒性.

科学领域:

生物医学工程生物医学工程
免疫学免疫学免疫学
材料科学材料科学材料科学

背景情况:

瘤相关巨细胞 (TAMs) 极大地影响免疫检查点阻塞 (ICB) 治疗结果.
将免疫抑制的M2 TAMs重新编程为促炎的M1 TAMs可以提高ICB的疗效.
系统性脂聚糖化物 (LPS) 的使用受到毒性限制,尽管它具有M1极化潜力.

研究的目的:

开发LPS局部药物输送系统,以克服其全身毒性.
研究LPS加载热敏水凝与ICB疗法结合的疗效.
评估基于水凝的LPS输送系统的免疫调节作用和安全概况.

主要方法:

制造热敏水凝 (GelF127CS-LPS) 通过将碳氧甲基基 (CS) 纳入Pluronic F127以进行局部LPS输送.
周注射GelF127CS-LPS给4T1瘤携带的小鼠,随后进行抗PD-1抗体治疗.
评估TAM表型 (M1/M2比),树突细胞激活,CD8+T细胞透,瘤生长抑制和存活率.
肝脏和血液学毒性的评估.

主要成果:

凝F127CS-LPS促进了局部LPS积累,将TAM重新编程为M1表型.
组合疗法显著增强了抗瘤效果,并改善了小鼠的存活率.
观察到瘤透树突细胞的激活增加和CD8+ T细胞透的增强.
治疗表明了有利的安全性,没有显著的肝脏或血液毒性.

结论:

凝F127CS-LPS水凝能够有针对性地输送LPS,在低剂量时有效调节TAM.
这种局部传递策略通过促进抗瘤免疫力,增强了ICB治疗的疗效.
开发的水凝代表了增强癌症免疫疗法的有希望的方法,并提高了安全性.

关键词:

癌症免疫疗法癌症免疫疗法提供LPS的交付方式.在TAMS重新编程过程中.热敏水凝是一种热敏水凝.

更多相关视频

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Published on: September 16, 2018

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.