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早期达到20-hydroxyecdysone值会影响蚊子在发育时间上的性变态

Meichun Zhang^1,2, Han Wen¹, Qiang Sun¹

¹Department of Microbiology, Genetics, & Immunology, Michigan State University, East Lansing, MI, USA.

Nature communications

|January 18, 2025

概括

蚊子达到临界体重取决于20-基 (20E) 值,影响化时间. 操纵20E水平可以通过扭曲性别比率来提供蚊子种群控制的策略.

科学领域:

昆虫学昆虫学是一门学科.
昆虫生理学昆虫生理学
分子内分泌学分子内分泌学

背景情况:

达到临界重量 (CW) 对于整体代谢昆虫的变形至关重要.
控制蚊子载体中CW评估和化时间的精确机制仍然不太清楚.
了解这些过程对于开发新的载体控制策略至关重要.

研究的目的:

调查20-基 (20E) 在临界重量 (CW) 评估和Aedes albopictus和Aedes aegypti的化时间中的作用.
阐明蚊子发育和化过程中性二态化的荷尔蒙基础.
探索操纵20E水平以控制蚊子种群的潜力.

主要方法:

在受控条件下培养阿尔博比克图斯和埃吉普蒂虫的幼虫.
在幼虫发育过程中量化20-基乙 (20E) 水平.
20E在特定发育阶段 (L3/L4 molt) 的实验性使用.
观察和记录化时间和生存率.

主要成果:

达到临界重量 (CW) 取决于达到特定的20-基 (20E) 值.
一个关键的20E值会触发一个精确的22小时间隔,导致20E脉冲的幼虫-虫生殖.
蚊子雄性表现出较高的基底20E水平,导致较早的CW达到和化比雌性.
在L3/L4变过程中对外部20E的使用导致了女性特异性的致死性,这表明治疗窗口狭窄.

结论:

20-基 (20E) 值在蚊子的临界重量 (CW) 确定,化时间和性二态性方面发挥着关键作用.
性别之间的基数20E水平差异显著导致观察到的化时间差异.
准20E水平是一个潜在的策略,可以将蚊子种群倾向于雄性,有助于媒介控制和疾病预防.

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