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一种系统的方法,用于可扩展的病毒样颗粒的净化
Enoch Y Park1, Robert Minkner2
1Laboratory of Biotechnology, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
Protein expression and purification
|January 19, 2025
概括
病毒样颗粒 (VLP) 作为疫苗和药物输送系统具有前景. 本次审查侧重于用于VLP生产的可扩展净化方法,解决下游加工当前的局限性.
科学领域:
- 生物技术是生物技术.
- 生物制药制造业 生物制药制造业
- 疫苗开发 疫苗开发
背景情况:
- 病毒样颗粒 (VLP) 对于疫苗和药物输送有价值,因为它们的非传染性和强烈的免疫性.
- 目前的VLP生产通常利用酵母和昆虫细胞等宿主,而丝虫作为一个具有成本效益的选择.
- 现有的VLP净化方法,主要是超离心,是不可扩展的,并显著增加生产成本.
研究的目的:
- 系统地审查可扩展的下游处理方法,用于VLP净化.
- 为应对当前VLP生产中的挑战,特别是需要高效和成本效益的净化.
- 为制药应用提供全面的概述,以指导未来的VLP生产.
主要方法:
- 对VLP净化下游可扩展流程发表的系统文献综述.
- 分析适用于封装和非封装VLP的方法.
- 专注于传统超离心法以外的方法.
主要成果:
- 确定并总结了各种可扩展的VLP净化技术.
- 强调下游加工对VLP整体生产成本和效率的重要性.
- 提供了基于VLP结构和属性的不同方法的适用性.
结论:
- 可扩展的净化对于推进用于制药用途的VLP生产至关重要.
- 开发高效的下游流程将降低VLP制造成本.
- 本综述为优化VLP净化策略提供了指导,并激发了进一步的研究.
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