鞭的流动诱导曲控制细菌的表面行为
Jessica-Jae S Palalay1, Joseph E Sanfilippo1
1Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA, 61801.
bioRxiv : the preprint server for biology
|January 20, 2025
概括
机械力量,而不仅仅是信号,控制细菌的表面行为. 剪切力曲鞭毛,影响了Pseudomonas aeruginosa的发生.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 流体动力学 流体动力学
背景情况:
- 细菌的表面行为通常归因于生物信号.
- 机械力量和尾重定向也可能发挥关键作用.
研究的目的:
- 为了研究剪切力诱导的鞭毛重定向如何控制Pseudomonas aeruginosa*的表面行为.
- 了解鞭毛旋转在流动下的细菌表面相互作用中的作用.
主要方法:
- 使用微流体和鞭毛标签用于高分辨率成像.
- 雇佣了带有的鞭毛突变体来评估旋转的影响.
- 在与宿主相关的剪切条件下分析单细胞行为.
主要成果:
- 旗旋转促进了在剪切下*Pseudomonas aeruginosa*的表面偏离.
- 根据鞭毛位置 (上游与下游) 出现了两个不同的细胞亚群.
- 上游的鞭毛被剪切曲,阻止旋转,而下游的鞭毛可以旋转,促进表面离开.
结论:
- 流和鞭毛之间的几何关系决定了细菌表面的行为和细胞命运.
- 机械力量,特别是剪切诱导的鞭毛重定向,产生亚群和控制细菌表面动态.
- 这种机制为细菌适应流动环境提供了新的理解.
相关概念视频
Mechanisms of Membrane-bending
2.6K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
2.6K
Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Mechanism of Ciliary Motion
3.6K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
3.6K
Mechanism of Lamellipodia Formation
2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Cytoskeletal Proteins in Bacteria
3.3K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.3K
Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K


