ATF6通过诱导胃素和改变胆固醇动态,使虫的病原体感染成为可能
Kaylee A Vosbigian1, Sarah J Wright1, Kristin L Rosche1
1Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA.
bioRxiv : the preprint server for biology
|January 20, 2025
概括
研究人员发现,的ATF6通路对于病原体的生存至关重要. 针对这种途径,特别是胃基因,通过控制胆固醇来限制病原体的生长,突出了载体能力的新方面.
科学领域:
- 分子生物学分子生物学
- 载体生物学 载体生物学
- 传播疾病 传播疾病
背景情况:
- 与宿主相互作用相比,我们对内的病原体相互作用,即疾病的载体,了解得很少.
- 展开的蛋白质反应 (UPR) 和它的ATF6分支在感染了Anaplasma phagocytophilum或Borrelia burgdorferi的身上被激活.
研究的目的:
- 为了研究ATF6通路在感染传播病原体的Xxodes scapularis中的作用.
- 为了识别和表征ATF6调节的基因,涉及到内的病原体生存.
主要方法:
- 开发一个自定义的R脚本 (ArthroQuest) 来分析ATF6绑定站点的tick促进器序列.
- 实验验证胃作为ATF6向基因,使用 luciferase 试验,药理活性剂和RNAi.
- 评估胃蛋白沉默对病原体殖民和宿主细胞胆固醇动态的影响.
主要成果:
- 鉴定出ATF6通路对于Ixodes中的Anaplasma phagocytophilum和Borrelia burgdorferi的生存至关重要.
- 斯托马丁被验证为直接ATF6点基因,参与脂质和胆固醇平衡.
- 静止胃氨酸会损害Anaplasma fagocytophilum的殖民性,并通过限制胆固醇的可用性来限制细菌的生长.
结论:
- 伊克索斯ATF6-ATF6通路通过通过胃基因调节胆固醇稳态,有助于载体能力.
- 通过ATF6调节胃似乎是血液养关节动物的独特特征,为载体控制提供了潜在的目标.
- 研究像虫这样的非模型生物体中的基本生物过程对于了解疾病传播至关重要.
相关概念视频
01:10Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
01:20The JAK-STAT Signaling Pathway
8.6K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.6K
01:20Infection
7.6K
When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
7.6K
01:20Receptor-mediated Endocytosis
6.0K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.0K