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重组,截断和水平转移塑造了细胞质不相容性模式的多样性

Alice Namias^1,2, Julien Martinez³, Iliana Boussou⁴

¹ISEM, Université de Montpellier, CNRS, IRD, EPHE, Montpellier, France.

bioRxiv : the preprint server for biology

|January 20, 2025

概括

在Culex pipiens蚊子中的Wolbachia细菌引起复杂的生殖变化. 单个全长cidB基因变异的身份,而不是截断的版本,似乎决定了这些细胞质不兼容性模式.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
进化生物学进化生物学

背景情况:

沃尔巴基亚是一种内共生细菌,以诱导细胞质不相容性 (CI) 等生殖操纵而闻名.
Culex pipiens蚊子表现出复杂的CI模式,与cidA和cidB基因的变异有关.
以前的研究表明基因变异和CI之间的相关性,但缺乏全面的解释.

研究的目的:

调查Wolbachia wPip菌株中的cid基因多态性是否可以完全解释和预测Culex pipiens中多样化的CI交叉模式.
从全球wPip菌株集合中分析完整的cid基因库.

主要方法:

通过使用一种全新的全基因获取方法,从全球45个wPip菌株中测序完整的cID谱.
分析cid基因多样性,包括重组和水平转移事件.
特定cidB基因变异与观察到的CI模式的相关性.

主要成果:

cid基因的广泛多样性源于重组和水平转移.
相互作用区域之外的cidB基因中的多态性与CI模式有很强的相关性.
唯一的全长cidB变异的身份,而不是截断的版本,决定了wPip菌株中的CI模式.
截断的cidB变种显示毒性和稳定性降低,可能会影响精子负载.

结论:

在Wolbachia wPip中,cid基因的多样性是由重组和水平基因转移形成的.
一个单一的全长cidB变体是Culex pipiens中细胞质不兼容性模式的关键决定因素.
截断的cidB变异可能在CI诱导中起到最小的作用,原因是功能受损.

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