肠道微生物组的表观遗传阶段变化增强了细菌的适应性
1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
bioRxiv : the preprint server for biology
|January 20, 2025
概括
表观遗传阶段变异 (ePV) 是人类肠道中常见的一种细菌适应策略. 这种DNA甲基化机制使肠道细菌能够调整基因表达,并在不断变化的环境中增强生存.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 人的肠道微生物组不断适应环境变化,如饮食和药物.
- 由DNA甲基化驱动的表观遗传相变 (ePV) 允许细菌菌株改变基因表达和表型状态.
- 对于ePV在肠道微生物群适应中的作用尚不清楚.
研究的目的:
- 在人类婴儿和成人肠道微生物组中全面编目全基因组和特定站点的ePV.
- 研究ePV在细菌适应环境波动中的作用.
- 了解ePV如何促进细菌的生存和耐受性.
主要方法:
- 长读元基因组测序以检测全基因组EPV和DNA甲基转移酶中的结构变异.
- 对公开的短读元基因组数据集的分析,以评估全基因组EPV的流行率.
- 单分子甲基化分析以定量检测特定站点的ePV和动态变化.
- 对*Akkermansia muciniphila*分离物的深入评估,以研究ePV对基因表达和抗生素耐受性的影响.
主要成果:
- 通过DNA甲基转移酶变异介导的全基因组ePV被检测到,特别是在抗生素使用和便微生物群移植方面.
- 在人类肠道微生物组中发现了全基因组EPV的高患病率.
- 确定了特定地点的ePV,显示了与抗生素治疗和益生菌干预相关的动态变化.
- 在 *Akkermansia muciniphila* 中的ePV被证明可以调节基因表达,并通过投注对冲增强适应能力,增加抗生素耐受性.
结论:
- 表观遗传修饰,特别是ePV,是肠道细菌适应的一个普遍策略.
- ePV使细菌菌株能够表现出表型异质性,并适应波动的环境条件.
- 了解ePV对于理解肠道微生物组动态和宿主微生物相互作用至关重要.
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