通过基于构成性促进物库的代谢工程来增强Botrytis cinerea中的酸生产
Ling-Ru Wang1, Ji-Zi-Hao Tang1, Shu-Ting Zhu1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, China.
Synthetic and systems biotechnology
|January 20, 2025
概括
研究人员为Botrytis cinerea开发了一种新的促进剂库,增强了酸 (ABA) 生产. 这一突破通过基因工程提高了ABA产量,改善了工业发酵过程.
科学领域:
- 生物技术是生物技术.
- 植物科学 植物科学
- 微生物发酵 微生物发酵
背景情况:
- 酸 (ABA) 是一种重要的植物生长调节剂,具有重要的农业和林业应用.
- 目前的工业ABA生产依赖于Botrytis cinerea发酵,但其有限的遗传工具阻碍了优化.
研究的目的:
- 为Botrytis cinerea开发一个新的构成性促进器库,以提高ABA生产.
- 通过促进剂工程和辅因子供应平衡来优化ABA生物合成.
主要方法:
- 从B. cinerea中选了10个构成性促进体,使用转录组分析和GUS活性测定.
- 通过使用Pef1a促进剂过度表达NADH激酶来设计辅因子供应.
- 通过在特定促进者 (Pthi4和Pef1a) 下过度表达HMGR和Bcaba4,实施推拉策略.
主要成果:
- 确定了一个具有跨越3-4个数量级的表达水平的促销商库.
- 通过Pef1a过度表达NADH激酶,ABA产量增加了32.26%.
- 工程菌株 (Pthi4:hmgr-Pef1a:a4) 实现了创纪录的ABA标位1.18g/L,增加了58.92%.
结论:
- 开发的构成性促进器库是B. cinerea的第一个,为基因操纵提供了有价值的工具.
- 促进器工程和代谢平衡显著提高了B. cinerea.中的ABA生产.
- 这项研究为改善工业ABA发酵过程提供了基础.
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