在Nibea coibor中隔离和评估使用益生菌利用酸盐生产细菌的潜在用途
Zhongzhen Li1,2,3,4,5, Ngoc Tuan Tran1,2,3, Ming Zhang1,2,3
1Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China.
Aquaculture nutrition
|January 20, 2025
概括
产生酸盐的细菌,如Clostridium butyricum和Proteocatella sphenisci可以改善水生动物的健康. 补充这些细菌,单独或与益生菌,增强了生长和免疫反应.
科学领域:
- 水生动物的健康和营养.
- 微生物学和免疫学 微生物学和免疫学
- 益生菌和益生菌和益生菌和益生菌.
背景情况:
- 酸盐产生细菌 (BPB) 对水生动物的福祉至关重要.
- 从鱼肠中识别和评估BPB是开发益生菌的关键.
- 尼比亚虫肠道微生物群的研究对于水产养殖的可持续性至关重要.
研究的目的:
- 为了从Nibea coibor肠道中分离和识别BPB.
- 评估隔离的BPB的益生菌潜力.
- 调查BPB,益生菌或它们的组合对N. coibor生长和健康的影响.
主要方法:
- 从N. coibor gut.中分离和体外培养细菌.
- 细菌分离物的鉴定,包括Clostridium butyricum和Proteocatella sphenisci.
- 在体内用BPB和益生菌 (GOS,胰岛素,D-sorbitol) 进行养试验,评估生长,肠道微生物群,短链脂肪酸 (SCFA) 和免疫参数.
主要成果:
- 获得了9个BPB分离物,包括C. butyricum和P. sphenisci.
- 用C. butyricum CG-3或P. sphenisci DG-1单独或与益生菌一起进行食补充,改善了N. coibor.的生长参数.
- BPB治疗调节了肠道微生物群,增加了SCFA的产生,并增强了免疫反应 (例如,IgM,TGF-β,IL-10),同时减少了氧化应激 (MDA).
结论:
- 在C. butyricum CG-3和P. sphenisci DG-1中显示出对N. coibor.具有显著的益生菌潜力.
- 这些BPB与GOS,胰岛素或D-sorbitol等益生菌的组合可以进一步增强生长和健康益处.
- 这些发现支持使用特定的BPB和益生菌来改善N. coibor水产养殖.
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