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相关概念视频

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.3K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Studying the Cytoskeleton01:17

Studying the Cytoskeleton

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
5.8K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

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相关实验视频

Updated: Jun 1, 2025

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy
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Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Published on: October 4, 2010

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使用原子力显微镜探测活细胞动力学和分子相互作用.

David Alsteens1,2

  • 1Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Croix du sud 4-5, L7.07.07, 1348 Louvain-la-Neuve, Belgium.

Biophysical reviews
|January 20, 2025
PubMed
概括
此摘要是机器生成的。

原子力显微镜 (AFM) 揭示了细胞表面机制和分子相互作用,如病毒与宿主结合. 这种技术提供了关于单分子水平的细胞过程和病毒进入机制的见解.

关键词:
原子力显微镜的原子力显微镜.动力力谱光学 动力力谱光学相互作用 相互作用结合体受体的结合体受体单分子力光谱学 单分子力光谱学

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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

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相关实验视频

Last Updated: Jun 1, 2025

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy
09:20

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Published on: October 4, 2010

11.3K
Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
08:41

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

39.9K
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

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科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 纳米技术纳米技术

背景情况:

  • 原子力显微镜 (AFM) 是单分子分析的关键技术.
  • 了解细胞和分子过程需要研究纳米级的相互作用.

研究的目的:

  • 审查生物应用AFM的进展.
  • 突出AFM在探测活细胞上的纳米机械性质和动态相互作用方面的能力.

主要方法:

  • 用于测量力和绘制分子相互作用的AFM.
  • 在近乎生理条件下的实地分析.

主要成果:

  • AFM捕捉了细胞表面的纳米机械特性.
  • AFM探测动态相互作用,包括病毒与宿主结合.
  • AFM提供了对细胞表面组织和受体功能的洞察.

结论:

  • 对于理解受体-连接体动态和病毒进入,AFM至关重要.
  • AFM在单分子水平上推进了细胞和分子过程的研究.