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可激活的红色/近红外水性有机光探头,用于改进时间分辨率生物成像

Yang Li¹, Zhiqin Wu¹, Zizhao Huang¹

¹Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.

National science review

|January 20, 2025

概括

研究人员开发了新的有机探针用于红色/近红外室温光成像 (RTP). 这些探测器使细胞和生物体的粘度在增强信号下能够实现无自光可视化.

科学领域:

超分子化学超分子化学
生物成像是一种生物成像.
有机电子学有机电子学

背景情况:

有机红色/近红外 (NIR) 室温光 (RTP) 提供无自光的生物成像,具有深层组织透.
开发可激活的红色/NIR有机RTP探头,用于可调节排放的水性环境是具有挑战性的.

研究的目的:

创建新的水性有机RTP探头,增强红色/NIR光强度和寿命.
为了证明这些探头对于先进的生物成像应用的实用性.

主要方法:

使用宏环[8]uril和含氨酸的基桥接化物制造超分子组件.
具有增强量子产量和寿命的粘度可激活光体的表征.
在 lysosomal粘度和时间分辨率细胞成像的双光子成像中应用.
在炎症小鼠中进行肠道光成像.

主要成果:

开发的探测器表现出粘度可激活的光,量子产量高达20%,寿命更长.
成功地实现了 lysosomal粘度的两光子成像和毫秒级的时间分辨率细胞成像.
与光成像相比,静脉内成像显示出更高的信号与背景比率,用于监测体内粘度变化.
超分子平台可实现多功能,高分辨率的光成像,用于体内追踪.

结论:

为可激活的红色/NIR有机RTP探针开发了一个新的超分子平台.
这些探测器为无自光生物成像提供了显著的优势,特别是在复杂的生物环境中.
该技术有望在体内监测粘度和其他具有高灵敏度和特异性的生理事件.

关键词:

可激活的分子探测器主人客人客人在体内生物传感生物感应.有机室温光是一种有机的光.时间分辨率成像技术

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