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在可重复使用的平台上使用酶和非酶方法快速检测氧化物和化物在造牛奶中

Harsha Bharwani¹, Suman Kapur¹, Sankar Ganesh Palani¹

¹Environmental Biotechnology Laboratory, Department of Biological Sciences, Birla Institute of Technology and Science Pilani, Hyderabad Campus, Jawahar Nagar, Kapra Mandal, Medchal District Hyderabad 500078 India sangan@hyderabad.bits-pilani.ac.in.

|January 20, 2025

概括

这项研究开发了一种快速的现场生物传感器套件,用于检测牛奶中的有害杂物过氧化 (HP) 和酸盐. 该套件提供了准确的检测,提高了食品安全和消费者健康保护.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
生物技术是生物技术.

背景情况:

牛奶经常被过氧化 (HP) 和酸盐等物质改,造成健康风险,如自由基失衡和甲基红蛋白血症.
添加HP是为了延长保质期,而酸盐污染通常来自用于增加牛奶量的水源.

研究的目的:

开发一个快速的,现场点的护理套件,用于检测常见的牛奶 adulterants:过氧化 (HP) 和酸盐.
创建稳定和可重复使用的生物传感器条带,以准确检测牛奶样本中的伪造物.

主要方法:

使用一种修改后的链条维丁-马过氧化酶 (Strep-HRP) -四甲基 (TMB) 试验来创建用于检测HP的生物传感器,将链条-HRP固定在聚烯条上.
用于检测亚酸盐,使用修改后的格里斯试验,涂层聚烯条带用由聚乙烯甘醇 (PEG) 稳定的格里斯试剂.

主要成果:

惠普生物传感器检测到的度为0.04% (v/v) 或更高,而酸盐生物传感器检测到32μg mL或更高.
开发的生物传感器条显示了25天 (HP) 和10天 (亚酸盐) 的稳定性,可重复使用 (3x HP,2x亚酸盐).
两条生物传感器带在检测真牛奶样本中的HP和酸盐方面都取得了高准确度,高达95%.

结论:

开发的点关怀套件提供了一种快速,准确和稳定的方法,用于在现场检测牛奶中的HP和酸盐.
这种生物传感器技术可以为确保牛奶质量和保护公众健康免受造乳制品的影响做出重大贡献.

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