希斯 lysine甲基转移酶及其特定的甲基化标记显示小鼠丸从年轻到年长的年龄有显著变化
Yesim Bilmez1, Gunel Talibova1, Betul Tire1
1Department of Histology and Embryology, Akdeniz University School of Medicine, Campus, 07070, Antalya, Türkiye.
Biogerontology
|January 20, 2025
概括
老化小鼠丸中的基因组甲基化变化,增加基因组 lysine 甲基转移酶 (KMTs) 和甲基化标记,有助于与年龄相关的生育能力丧失. 老化丸中减少的亡也可能起作用.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 老年学是一门学科.
背景情况:
- 精子生成通过基因组甲基化进行表观遗传调节,涉及关键的基因组 lysine 甲基转移酶 (KMTs).
- 特定的KMT (SETD1B,CFP1,SETDB1,G9A,SETD2) 建立了在精子发生过程中对基因表达和染色质重塑至关重要的基因基因甲基化标记 (H3K4me3,H3K9me2,H3K9me3,H3K36me3).
研究的目的:
- 为了研究KMTs的时空表达及其相关的素甲基化标记在老化过程中的小鼠丸.
- 为了评估与衰老相关的β-galactosidase (β-GAL) 活性,转录活性和老鼠丸中亡率的变化.
主要方法:
- 根据年龄分组Balb/C小鼠:年轻 (1-2周),青春期前 (3-4周),青春期 (5-6周),青春期后 (16-20周) 和老年 (48-52周).
- 评估了β-GAL染色,SETD1B,CFP1,SETDB1,G9A和SETD2的蛋白质水平,以及H3K4me3,H3K9me2,H3K9me3和H3K36me3.3的水平.
- 评估了不同年龄组的精卵管中的亡率.
主要成果:
- 随着年龄的增长,β-GAL染色显著增加 (P < 0.01).
- 在老年小鼠的精子和塞尔托利细胞中,SETD1B,G9A,SETDB1和SETD2的蛋白质水平增加 (P <0.05),而CFP1水平下降 (P <0.05).
- 在老丸中,H3K4me3,H3K9me2,H3K9me3和H3K36me3的水平升高 (P <0.05),随之而来的是亡率显著降低 (P <0.01).
结论:
- 由于老年小鼠丸中KMT表达增加,基因组甲基化标记的积累可能会破坏染色体重编程和基因表达.
- 这些表观遗传变化可能会导致与年龄相关的丸功能和生育能力下降.
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