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hsa_circ_0021727通过与EIF4A3相互作用稳定GBX2mRNA,促进食道状细胞癌的进展

Jie Lin¹, Qiuping Zhu¹, Fanlin Zeng²

¹Department of Intensive Medicine/Comprehensive Intensive Care Unit, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.

|January 20, 2025

概括

循环RNA hsa_circ_0021727 (circ_0021727) 通过稳定胃流动大脑本源盒2 (GBX2) mRNA,促进食道状细胞癌 (ESCC). 向circ_0021727可能为ESCC提供新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

食道状细胞癌 (ESCC) 是一个重大的全球卫生挑战,死亡率高.
循环RNA的特定作用和调控机制,如hsa_circ_0021727 (circ_0021727),在ESCC病原发生过程中尚不清楚.

研究的目的:

研究circ_0021727对ESCC细胞周期进展,细胞亡和血管生成的影响.
阐明circ_0021727在ESCC中的功能背后的监管机制.

主要方法:

定量实时PCR用于评估circ_0021727的表达水平.
道测定和流动细胞计量来评估细胞亡和细胞循环.
管状形成测试用于评估血管生成.
RNA免疫沉 (RIP) 和RNA拉下测试以确定分子相互作用.
瘤异种移植模型在体内.

主要成果:

在ESCC细胞中,Circ_0021727被显著上调.
敲除circ_0021727诱导G0/G1阶段停止,促进细胞亡,并抑制ESCC细胞中的血管生成.
Circ_0021727 直接结合胃化大脑本源盒2 (GBX2) mRNA和真核细胞翻译启动因子4A3 (EIF4A3),增强GBX2 mRNA的稳定性.
过度表达GBX2逆转了circ_0021727对ESCC进展的抑制作用.
在体内研究证实,circ_0021727敲击抑制了瘤生长,这种效应被GBX2过度表达所抵消.

结论:

通过招募EIF4A3.3,Circ_0021727通过增加GBX2mRNA稳定性来促进ESCC的进展.
这种机制增强了ESCC中的细胞循环进展和血管生成.
Circ_0021727代表了食道状细胞癌的潜在治疗标.

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