相关实验视频

Updated: Jun 1, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

circ_0075829通过miR-330-5p/TCF4轴调节结肠癌细胞中的铁和免疫逃逸

Huajun Fan¹, Yu Ding², Zhe Xiao³

¹Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuchang, Wuhan, Hubei, China.

|January 20, 2025

概括

像circ_0075829这样的循环RNAs (circRNAs) 调节结肠癌中的铁和免疫逃生. 这项研究表明,circ_0075829通过海绵miR-330-5p促进结肠癌,以调节TCF4,影响细胞死亡和免疫反应.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

循环RNAs (circRNAs) 越来越多地与结肠癌的发展和进展有关.
了解circRNAs在癌症相关过程中的特定作用,如铁亡和免疫逃逸,对于治疗的发展至关重要.

研究的目的:

调查circ_0075829在铁亡和结肠癌中的免疫逃脱中的调节机制.
为了阐明涉及circ_0075829,miR-330-5p和TCF4在结肠癌进展中的分子途径.

主要方法:

利用结肠癌细胞系和异种移植小鼠模型进行体外和体内分析.
通过qRT-PCR和西式涂抹评估基因和蛋白质表达.
使用双露西法酶记者和RNA下拉试验研究了分子相互作用;评估了铁亡标记物和免疫细胞反应.

主要成果:

抑制circ_0075829诱导的铁亡 (增加Fe2+,ROS,MDA;减少GPX4,xCT) 和抑制免疫逃逸 (增强CD8+T细胞增殖,增加IFN-γ,IL-2,TNF-α;减少PD-L1).
circ_0075829 作为miR-330-5p的分子海绵,导致TCF4上调.
证实TCF4是miR-330-5p的下游目标;调节miR-330-5p或TCF4可以逆转circ_0075829的淘汰效应.

结论:

circ_0075829显著调节结肠癌中的铁和免疫逃生.
该机制涉及circ_0075829海绵式miR-330-5p调节TCF4表达,从而影响结肠癌的进展.

更多相关视频

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Receptor Downregulation in MVBs

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...

Adaptive Mechanisms in Cancer Cells

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...

Loss of Tumor Suppressor Gene Functions

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...