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来自不同商业乳清蛋白材料的N-甘氨酸的结构和含量的比较分析

Wanyi Zhang¹, Qiuqi Peng¹, Linhan Yuan¹

¹College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin, China.

Journal of food science

|January 20, 2025

概括

这项研究分析了用于婴儿配方奶粉的商业乳清蛋白材料中的N-甘氨酸. 牛乳酸和WPC 70的产量显示出基化和基化N-甘氨酸的最高产量.

科学领域:

食品科学食品科学食品科学
葡萄糖生物学葡萄糖生物学
乳制品化学乳制品化学

背景情况:

甘氨酸是婴儿配方奶粉中的功能成分,但它们的潜力仍未得到充分利用.
商业乳清蛋白质材料是婴儿配方奶粉的关键成分,需要分析它们的N-糖甘配置文件.
了解乳清蛋白中的N-甘氨酸对于优化婴儿配方奶粉成分至关重要.

研究的目的:

分析和描述各种商业乳清蛋白材料中存在的N-甘氨酸.
在不同的乳清蛋白源中识别特定的N-甘氨酸结构,包括福克西化和化类型.
量化N-甘氨酸含量,并比较选择的乳清蛋白材料中的化和化水平.

主要方法:

使用矩阵辅助飞行质谱仪 (MALDI-TOF-MS) 的激光脱/离子化时间进行N-甘分析.
采用离子染色学来进一步描述和量化乳清蛋白样本中的N-甘氨酸.
在牛乳酸 (Lf),乳清蛋白分离物 (WPI),乳清蛋白缩物70 (WPC 70),山羊乳清蛋白粉50 (D90),非矿化乳清粉90 (D90) 和脱盐山羊乳清粉中研究的N-甘氨酸.

主要成果:

在不同的乳清蛋白源中检测到不同数量的N-甘氨酸 (例如,在Lf中30个,在D90中6个,在脱盐的山羊乳清粉中28个).
总共确定了四种不同的N-甘氨酸结构,其中基化和基化结合 (SFN类型) 结构仅在牛Lf和WPC 70中发现.
在WPC 70中,N-甘氨酸产量最高 (14.5 mg/g),与化相比,化度更高.

结论:

商业乳清蛋白材料含有多种不同的N-甘氨酸配置文件,来源之间存在显著差异.
牛肉Lf和WPC 70特别丰富于特定的N-甘氨酸结构,适用于功能应用.
这项研究为商业乳清蛋白材料在乳制品,包括婴儿配方奶粉中有针对性的使用提供了基础.

关键词:

在N-glycan中使用.WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 WPC 70 商业乳清蛋白是商业乳清蛋白.内容内容内容内容内容内容内容内容.结构的结构结构的结构.

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