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一种藻类 (Raphidocelis subcapitata) 的转录基因反应暴露于22个per-和多甲基物质

Kevin M Flynn¹, Kendra Bush², Jenna Cavallin¹

¹United States Environmental Protection Agency, Center for Computational Toxicology and Exposure, Great Lakes Toxicology and Ecology Division, Duluth, MN, United States.

Environmental toxicology and chemistry

|January 20, 2025

概括

转录基因起点 (tPOD) 用于评估中的和多基物质 (PFAS) 毒性. 这种方法识别了藻类tPOD,为许多PFAS化学品的生态危害评估提供了有希望的方法.

科学领域:

环境毒理学环境毒理学
生态毒理学生态毒理学
分子生物学分子生物学

背景情况:

和多基基物质 (PFAS) 是广泛存在的环境污染物,生态危害数据有限.
现有的毒性测试方法不足以评估数千种已识别的PFAS的风险.
对PFAS的生态危害数据很少,这阻碍了全面的风险评估.

研究的目的:

调查转录基因起点 (tPODs) 对评估藻类中PFAS毒性的有用性.
使用淡水绿藻物种,为22种不同的PFAS建立tPOD.
为了将藻类tPOD与其他种类的现有危险基准进行比较.

主要方法:

暴露Raphidocelis subcapitata (藻类) 在微板中的22个PFAS24小时.
提取RNA并执行RNA测序以分析转录组.
应用度响应建模来确定每个PFAS的tPOD.

主要成果:

确定了22个PFAS的tPOD,范围从0.9μg/L ( perfluorotridecanoic酸) 到1mg/L ( perfluorononanoic酸).
与其他测试的种类和体外测试相比,Raphidocelis subcapitata通常对PFAS表现出更高的敏感性.
藻类的tPOD明显低于大湖支流中发现的最大PFAS度 (大约3个数量级).

结论:

使用藻类的高通量转录组学为PFAS的生态危害评估提供了一个有希望的方法.
来自藻类转录组的tPOD可以为大量PFAS提供分层危险评估策略.
这种方法提供了一种更有效的方式来生成生态危害数据,用于对新出现的污染物的风险评估.

关键词:

藻类是一种藻类.水生毒理学水生毒理学通过和多基基物质.起点的起点是出发点.翻译学翻译学翻译学翻译学

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