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设计一个可开启的光探针,用于在环境和生物系统中可视检测HOCl

Meng-Qian Zhang¹, Wen-Hua Guan², Jiahe Wang¹

¹School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|January 20, 2025

概括

研究人员开发了一种新的光探针,OX-OCl,用于快速和特定检测低酸 (HOCl). 这种探测器可以实时监测环境样本,并在活细胞中进行生物成像,以诊断疾病.

科学领域:

分析化学分析化学
生物医学工程生物医学工程
环境科学环境科学

背景情况:

低酸 (HOCl) 在生物系统中起着双重作用,维持正常水平的氧化还原平衡,但在过度度下会造成伤害.
准确检测HOCl对于理解其生理作用和监测环境污染至关重要.

研究的目的:

为了设计和评估一个新的,HOCl特定的自燃光探针 (OX-OCl).
开发用于环境和生物样本中实时HOCl监测的传感平台.
评估探测器在生物成像中对疾病诊断的实用性.

主要方法:

基于N-保护的氧染料的OX-OCl探针的合成和表征.
在水溶液中评估探头反应动力学,特异性和灵敏度.
开发智能手机辅助的色度和光传感平台.
使用活细胞和小鼠模型进行体外和体外生物成像研究.

主要成果:

OX-OCl探针对HOCl表现出快速 (15秒) 和高度特异性的反应,检测极限低 (15.3 nM).
在与HOCl反应时,观察到紫外线吸收和光发射的显著变化.
基于智能手机的平台实现了实时HOCl检测.
由于探针的生物相容性,在活细胞和关节炎小鼠模型中成功实现了HOCl的近红外生物成像.

结论:

OX-OCl 探针为HOCl检测提供了一种敏感和选择性的方法.
开发的传感平台为环境监测和早期临床诊断HOCl相关疾病提供了方便的工具.
这种探测器代表了HOCl分析在各种应用中的重大进步.

关键词:

光探测器探测器可以发光.HOClCl HOClCl 的时间.近红外生物成像近红外生物成像可携带的视觉传感平台可携带的视觉传感平台自焚是一种自焚.

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