内在的双发射Si点用于高精度和广泛的pH检测.
Chunmin Qiu1, Guoqiang Wu2, Puying Zhao3
1College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China; Shenzhen Key Laboratory of Synthetic Genomics, Guangdong Provincial Key Laboratory of Synthetic Genomics, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Analytica chimica acta
|January 20, 2025
概括
研究人员开发了简单的双发射点,用于精确的pH监测和尿酶检测. 这些光探针为健康评估中的生物传感和生物成像提供了一个多功能平台.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 分析化学 分析化学
背景情况:
- 精确的pH监测对于了解细胞过程和评估健康状况至关重要.
- 现有的响应pH的光探针往往涉及复杂的合成,并遭受效率和稳定性降低.
- 需要简单,稳定和高度敏感的pH检测方法.
研究的目的:
- 开发一种用于制备双排放点 (Si点) 的简单方法,用于高精度,广泛的pH监测.
- 探索这些Si点在检测尿酶活性中的应用.
- 为了证明Si点对活细胞pH成像的有用性.
主要方法:
- 内在双发射Si点的合成,其发射中心在427nm和500nm.
- 在广泛的pH范围 (3.0-12.0) 中,Si点光反应的表征.
- 使用Si点根据pH值变化量化尿酶活性.
- 在HeLa细胞中的体内pH值成像.
主要成果:
- 点在427nm和500nm处表现出明显的光变化,以响应pH值变化.
- 在7.0-9.5和10.0-12.0的范围内实现了高精度的线性pH检测.
- 尿酶检测表明线性范围为1-80 U/L,低检测极限为0.28 U/L.
- 在活着的HeLa细胞中成功进行pH成像,显示与细胞内pH转移相对应的颜色变化.
结论:
- 开发的内在双发射Si点为与pH相关的生物传感和生物成像提供了一个简单而通用的平台.
- 这种方法提供了一种可靠的方法,用于准备双排放探头和构建光比度,以评估健康状况.
- 点显示出在生物和医学领域的应用有很大的潜力,需要精确的pH监测.
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