代谢活动是个别细分时钟过程的选择性调节器
Mitsuhiro Matsuda1,2, Jorge Lázaro3,4, Miki Ebisuya5,6,7
1European Molecular Biology Laboratory (EMBL) Barcelona, Barcelona, Spain. mitsuhiro.matsuda@tu-dresden.de.
Nature communications
|January 20, 2025
概括
代谢活动选择性调节胚胎发育的细分时钟,而不是全球. 不同的代谢抑制独特地影响Hes7基因表达和降解,这表明进化约束塑造了这些选择性调节器.
科学领域:
- 发育生物学是发展生物学.
- 细胞和分子生物学是细胞和分子生物学.
- 系统生物学 系统生物学
背景情况:
- 胚胎发育涉及协调的细胞和分子过程.
- 细分时钟由Hes7基因动力学调节,对胚胎发育至关重要.
- 了解节奏协调和全球调制器是一个基本问题.
研究的目的:
- 为了调查代谢活动是否在全球范围内调节细分时钟节奏.
- 确定代谢过程对时钟基因动力学的选择性与全球影响.
主要方法:
- 抑制关键的代谢途径,包括糖解和电子运输链.
- 分析Hes7基因表达,蛋白质降解和内部延迟动力学.
- 检查联合代谢抑制的协同效应.
主要成果:
- 代谢抑制选择性地影响细分时钟过程,而不是作为全球调节器.
- 抑制糖解会减缓Hes7蛋白质的降解,并延迟生成,但不会延迟内部.
- 电子运输链的抑制延长了内部延迟,而不会影响其他Hes7动力学.
结论:
- 代谢活动是细分时钟的选择性调节器,而不是全局调节器.
- 跨物种细分时钟的缩放动力学可能源于由进化塑造的组合选择性调节器.
- 一个单一的全球调制器不太可能解释细分时钟过程的协调.
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