Piezo1通过调节NF-κB/p65轴促进振动诱导的血管光滑肌损伤
Yingshan Zeng1,2, Zhiquan Wu1,2, Mengtian Xiong1,2
1Department of Occupational and Environmental Health, School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China.
Communications biology
|January 20, 2025
概括
职业接触手传动振动 (HTV) 可能导致手臂振动综合征 (HAVS). 这项研究揭示了Piezo1和NF-κB/p65通路驱动振动诱导的血管损伤,提供潜在的治疗点.
科学领域:
- 机械生物学 机械生物学
- 职业健康 职业健康 职业健康
- 血管生物学 血管生物学
背景情况:
- 手臂振动综合征 (HAVS) 与职业接触手传动振动 (HTV) 有关.
- 外周循环系统损伤是HAVS的早期指标.
- 振动诱导的血管损伤的精确机制需要进一步阐明.
研究的目的:
- 调查机械敏感通道Piezo1在振动诱导的血管损伤中的作用.
- 探索Piezo1和NF-κB/p65信号通路在血管功能障碍中的关联.
- 为了确定早期HAVS诊断的潜在生物标志物.
主要方法:
- 利用老鼠振动模型和蜂振动模型来模拟职业HTV暴露.
- 评估了血管功能,Piezo1表达和NF-κB/p65通路激活的变化.
- 采用Piezo1敲击和p65抑制技术来评估它们对振动诱导效应的影响.
主要成果:
- 在动物和细胞模型中,振动暴露增加了Piezo1的表达,并诱导了血管功能障碍.
- 振动导致通过NF-κB/p65通路通过Piezo1介导的血管性化学因子 (CCL2,CCL5,CXCL1,CXCL2,CXCL10) 的上调.
- 抑制Piezo1或p65减弱振动诱导的血管功能障碍.
结论:
- 皮埃佐1-NF-κB/p65轴在振动诱导的血管功能障碍中起着至关重要的作用.
- 化学因子被确定为HAVS.早期诊断的潜在生物标志物.
- 皮埃佐1和NF-κB/p65通路代表了HAVS.的有希望的治疗点.
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