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Zhihua Li^1,2,3, Chi Zhao⁴, Zhenyu Mao^5,6

¹Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China. lzh82@hotmail.com.

Environmental microbiome

|January 20, 2025

概括

坑坑泥 (PM) 中的微生物群落经历了三个阶段的进化,乳酸菌在早期占主导地位,甲基生物/密室菌在晚些时候. 水平基因转移 (HGT) 是PM的关键.

科学领域:

微生物学微生物学
环境科学环境科学
生物技术是生物技术.

背景情况:

坑泥 (PM) 拥有独特的微生物生态系统,对Baijiu生产至关重要.
颗粒微生物对乙醇和酸具有耐受性,形成一个独特的环境.
对微粒微生物生态和代谢功能,特别是新型血统的理解有限.

研究的目的:

综合分析微生物群落结构和空间时间PM样本中的代谢功能.
为了研究分化,横向基因转移 (HGT) 和与PM中的碳素化合物的相互作用.
阐明PM生态系统中的生态动态和进化途径.

主要方法:

对微生物群落进行基因组学分析.
化学衍生液态染色学-质谱学 (LC-MS) 用于代谢物分析.
基因组组合基因组 (MAG) 的构建和遗传学分析.

主要成果:

在PM中的 Prokaryotic 社区表现出三个时空阶段:初始 (以 Lactobacillus 主导),过渡期和平衡期.
确定了178种高质量的MAG,涉及碳,和硫循环,并通过Wood-Ljungdahl和rTCA途径固定碳.
观察到广泛的HGT,显著影响发酵,碳固定,甲生产和营养循环.

结论:

PM微生物群体通过不同的发育阶段进化,受到环境因素和HGT的影响.
乳杆菌,甲基生物和Clostridium动态是Baijiu生产PM质量的关键指标.
了解空间时空的PM生态和微生物相互作用为发酵过程和质量评估提供了洞察力.

关键词:

炭基代谢物的代谢物.进化上的分歧.横向基因转移是指水平基因转移.超基因组组装的基因组组合在一起.坑坑的泥坑坑的泥

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