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Updated: Jun 25, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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通过使用纳米混合膜进行大规模样本聚合来提高RT-PCR吞吐量和灵敏度

Na Eun Lee1,2, Kang Hyeon Kim1, Ji Hye Hong1,3

  • 1Department of Electrical Engineering, Kwangwoon University, Seoul, 01897, Republic of Korea.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|January 21, 2025
PubMed
概括

这项研究引入了一种新的纳米混合膜方法,以提高聚合样本的RT-PCR精度. 它防止病毒稀释,保持精确检测SARS-CoV-2和流感的敏感性.

关键词:
在SARS-CoV-2中.测试组测试 测试组测试 测试组测试高通量RT-PCR高通量RT-PCR的使用方法样本丰富的方法抽样组合 抽样组合 抽样组合

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...