相关实验视频
Updated: Jun 1, 2025

11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
9.4K
表皮-半机体过渡功能作为 somatic 细胞直接转换的驱动器
Tsutomu Motohashi1, Hitomi Aoki2, Takahiro Kunisada2
1Department of Molecular Design and Synthesis, Functional Biology Division, Gifu University Graduate School of Medicine, Gifu, Japan.
Stem cells and development
|January 21, 2025
概括
直接转化绕过多能性,用于细胞重编程. 诱导表皮层-介质细胞转换 (EMT) 显著提高直接转换效率,显示出再生医学的前景.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 再生医学是一种再生医学.
背景情况:
- 直接转化旨在将体细胞重新编程为目标细胞,而不需要多能阶段.
- 转录因子 (TFs) 在重编程过程中对指导细胞命运至关重要.
- 当前的方法在实现高效的直接转换方面经常遇到局限性.
研究的目的:
- 调查表皮层-介质细胞转换 (EMT) 在增强直接细胞转换中的作用.
- 为了确定诱导EMT是否提高了将体细胞转化为特定目标细胞的效率.
主要方法:
- 强制表达特定的转录因子 (TF),包括Sox10.
- 在体细胞中使用TFs (Snail1, Slug, Twist1, Tcfap2a) 或TGF-β1.1.a组合诱导EMT.
- 评估质细胞和NMuMG细胞直接转化为神经细胞 (NCC).
主要成果:
- 通过TFs诱导EMT增强了小鼠角质细胞的直接转化为NCCs.
- 在与Sox10表达相结合时,EMT诱导是直接将NMuMG细胞转化为NCC所必需的.
- 在TGF-β1诱导的EMT之后,Sox10表达成功地将NMuMG细胞转化为NCC.
结论:
- 表皮-介质细胞转换 (EMT) 是直接细胞转换的优势.
- 诱导EMT可以克服直接转换的障碍,提高效率.
- 这一发现对推进细胞重编程技术具有重要意义.
相关概念视频
Forced Transdifferentiation
1.9K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
Artificial...
1.9K
Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Mesenchymal Stem Cells
4.6K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.6K
Metastasis
5.5K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.5K
Cadherins in Tissue Organization
2.9K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
2.9K
Induced Pluripotent Stem Cells
3.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
3.9K

