瘤微环境中的单细胞菌模式调节细胞间通信,影响肝细胞癌的进展和预后
Zhengyan Li1, Wei Chen1, Shu Yao1
1Department of Hepatobiliary Surgery, Daping Hospital, Army Medical University, Chongqing, China.
Frontiers in immunology
|January 21, 2025
概括
肝细胞癌 (HCC) 中的线粒会影响细胞通信和患者的存活率,但不会影响免疫治疗的有效性. 准线粒可能会改善HCC患者的治疗结果.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 肝细胞癌 (HCC) 是一种常见的消化系统恶性瘤,具有显著的死亡率.
- 目前的治疗方法,包括免疫疗法,面临着局限性,导致患者的治疗结果低于最佳.
研究的目的:
- 为了研究 mitoophagy 在肝细胞癌瘤微环境 (TME) 中的作用.
- 探索线粒对细胞间通信,瘤进展和HCC患者预后的影响.
主要方法:
- 从HCC患者 (25,189个细胞) 的两个单细胞RNA测序 (scRNA-seq) 数据集的分析.
- 在癌症相关纤维细胞 (CAF),T细胞,B细胞和瘤相关巨细胞 (TAMs) 中利用非负矩阵因子化 (NMF) 进行线粒细胞衰变模式识别.
- 使用CellChat进行细胞间通信分析,使用Monocle进行伪时间轨迹分析,使用SCENIC进行基因调控网络调查.
主要成果:
- 在HCC样本中确定了22个和7种主要细胞类型,揭示了复杂的细胞间通信.
- 在CAFs,TAMs,CD8+ T细胞和B细胞中发现了与mitophagy相关的亚型,这些亚型表现出不同的生物状态和代谢途径.
- 在瘤样本中观察到较高的线粒细胞衰竭水平,线粒细胞衰竭基因的改变与总生存率相关,但与免疫疗法的疗效无关.
结论:
- 在HCC TME内的线粒显著调节细胞间通信,影响瘤进展和患者预后.
- 向线粒菌是一种潜在的治疗策略,可以改善HCC患者的长期治疗结果.
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