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病毒模拟细菌外膜囊泡用于向 ангиотензин转化酶2

Gna Ahn^1,2, Hyo-Won Yoon¹, Ju Hwan Jeong^3,4

¹Department of Microbiology, Chungbuk National University, Cheongju, Republic of Korea.

International journal of nanomedicine

|January 21, 2025

概括

工程外膜囊泡 (OMVs) 显示SARS-CoV-2受体结合域 (RBD) 用于theranostic应用. 这些病毒模拟OMV显示出对新兴传染病的诊断,疫苗和治疗的前景.

科学领域:

纳米技术纳米技术
微生物学微生物学
免疫学免疫学免疫学

背景情况:

来自格拉姆阴性细菌的外膜囊泡 (OMV) 是用于诊断,疫苗和治疗的多功能纳米平台.
精制人体治疗应用的OMV需要针对特定疾病应用进行有针对性的修改.

研究的目的:

开发一个最小的模块化系统,使用工程 OMV 显示 SARS-CoV-2 的受体结合域 (RBD).
为了创建病毒模拟Ag43β700_RBDOMV,针对血管酶转化酶2 (ACE2) 进行治疗应用.

主要方法:

构建的 E.E. 大肠杆菌衍生OMV通过抗原43自传输系统 (Ag43β700_RBD OMV) 显示RBD.
通过以洗剂为基础的膜破坏制造的OMV并使用连续离心法净化,产生球状囊泡 (平均直径218 nm).
在使用素治疗的OMV上确认了RBD表达.

主要成果:

Ag43β700_RBD OMVs在ACE2阻断和ELISA测定中和抗体 (nAbs) 的量化中有效.
在体外,OMVs降低了促炎性细胞因子 (IL-6,IL-1β).
在小鼠中,Ag43β700_RBD OMVs证明了安全性,诱导了nAbs (39-58%的中和),并减少了肺和大脑中的病毒载荷,而不会导致体重减轻.

结论:

开发的病毒模仿Ag43β700_RBDOMVs作为一个有前途的纳米微丝-theranostic平台.
这个平台有可能在未来用于诊断,接种疫苗和治疗新出现的传染病.

关键词:

血管氨酸转化酶 2 2 抗原43自传输体的自传输体外膜囊泡的外膜囊泡是什么?有针对性的送货车辆.他们是天文学家.

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