在使用农业工业废物进行酵母介导的浸泡发酵中生产和表征脂
Aishwary Purohit1,2, Amar Jyoti Das1, Debashish Ghosh2,3
1Department of Microbiology, Graphic era Deemed to be University, Dehradun 248002, Uttarakhand, India.
Current research in microbial sciences
|January 21, 2025
概括
这项研究表明,使用Rhodotorula mucilaginosa从甘包废物中生产脂生物表面活性剂的环保生产. 优化的浸泡发酵实现了2.6g/L的产量,证实了可持续生物表面活性剂应用的潜力.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 微生物学 微生物学
背景情况:
- 农业工业废物管理带来了重大的环境挑战.
- 生物转化为废物的价值化提供了可持续的解决方案,使废物变成有价值的产品.
- 从废物中生产生物表面活性剂在成本效益和环保性方面越来越重要.
研究的目的:
- 研究从农业工业废物中生产脂生物表面活性剂的方法.
- 为了优化水下发酵,使用甘包水解剂.
- 描述所产生的脂和确认它们的结构.
主要方法:
- 用于浸泡发酵的使用的Rhodotorula mucilaginosa IIPL32.
- 作为低成本原料,采用了甘包水解剂.
- 优化了应变适应性,中等成分,并从震动瓶扩展到生物反应器.
- 进行了广泛的表征,包括乳化,表面张力降低,化学分析,FTIR,LC-MS和NMR光谱学.
主要成果:
- 达到2.6 ± 0.21 g/L的最大脂产量.
- 显示显著的表面张力降低和乳化指数为54%.
- FTIR,LC-MS和NMR光谱学证实了这种结构是具有不同脂质链长度和乙化的脂质.
结论:
- 农业工业废物,特别是甘包水解剂,是环境友好的脂生物表面活性剂生产的可行原料.
- 优化发酵和扩大规模对于高效的生物表面活性剂产量至关重要.
- 描述的脂体显示出在制药,化品和环境修复等领域的各种应用潜力.
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