相关实验视频

Updated: Jun 1, 2025

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Published on: May 10, 2021

非规范性TERT活动启动骨质发生在动脉疾病中的骨质发生

Rolando A Cuevas¹, Luis Hortells², Claire C Chu¹

¹Division of Cardiology, Department of Medicine, Pittsburgh Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA (R.A.C., C.C.C., R.W., A.C., C.K.B., C.R., W.J.M., M.J. Bashline, A.P., A.M.P., P.B., M.J.B., C.S.H.).

Circulation research

|January 21, 2025

概括

与端粒相关的酶端粒酶逆转录酶 (TERT) 通过非正规活动启动骨质性重编程来驱动动动脉疾病. 抑制TERT及其合作伙伴STAT5为血管化提供了一个潜在的治疗策略.

科学领域:

心血管生物学心血管生物学
分子生物学分子生物学
细胞重编程细胞重编程

背景情况:

动脉疾病涉及心脏门的病态重塑.
门叶片骨质性重编程的早期阶段尚未完全理解.
端粒酶逆转录酶 (TERT) 过度表达原始干细胞用于骨质分化.

研究的目的:

研究TERT在膜间歇细胞骨质性重编程中的作用.
确定TERT是否有助于性大动脉膜疾病的病原发生.
确定血管化新型治疗点.

主要方法:

使用了人类大动脉的说明书和来自患者的间歇细胞,用于体外和体外化试验.
在小鼠中使用TERT敲除和遗传删除进行了功能丧失研究.
在模拟,共免疫沉和ChIP-seq中使用,以确定TERT相互作用和DNA结合.

主要成果:

在化门中,TERT蛋白的表达很高,独立于端粒长度或衰老.
随着骨质性/炎症性刺激,TERT表达增加;TERT抑制可以防止化.
TERT通过非正规活动启动了骨质再生编程,与STAT5相互作用,结合RUNX2促进体.

结论:

TERT的非正规活动对于启动形大动脉膜疾病至关重要.
炎症上调的TERT与STAT5合作调节RUNX2,这是骨质再生重编程中的关键基因.
准TERT-STAT5-RUNX2通路为血管化提供了一个新的治疗策略.

关键词:

大动脉门的大动脉门这是一种炎症炎症炎症炎症.骨质发生过程 (osteogenesis)血管化血管化

更多相关视频

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Published on: November 20, 2017

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

Published on: February 4, 2021

相关概念视频

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...