再组合FSH通过部分调节人类和小鼠颗粒细胞中的let-7表达来诱导孕激素
概括
重组卵泡刺激激素 (rFSH) 可以增加孕激素,可能降低体外受精 (IVF) 的成功率. 这项研究确定了let-7微RNA作为颗粒细胞中rFSH诱导的孕激素产生的关键调节剂.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
背景情况:
- 在体外受精 (IVF) 周期期间过早的孕激素升高可以通过降低子宫内膜受体性来对活产率产生负面影响.
- 复合毛囊刺激激素 (rFSH) 的使用与孕激素水平的增加有关,但潜在的机制需要进一步研究.
研究的目的:
- 调查FSH在颗粒状细胞中孕激素生产中的作用.
- 探索涉及let-7微RNA在rFSH诱导的孕激素升高中的潜在机制.
主要方法:
- 实时RT-PCR被用于评估人类积分细胞和小鼠颗粒细胞中的let-7表达.
- 用不同度的rFSH.对待 (人类和小鼠) 颗粒细胞.
- 使用let-7模仿剂和抑制剂来评估它们对孕激素水平和rFSH反应的影响.
主要成果:
- 来自高孕激素水平患者的人类累积细胞显示LET-7a表达减少.
- rFSH治疗增加了孕激素水平,并降低了人类和小鼠颗粒细胞中的let-7a表达.
- 调节let-7a表达直接影响孕激素水平,模仿剂增加let-7a并降低孕激素,抑制剂显示相反的效果.
结论:
- let-7微RNA在调节人类和小鼠颗粒细胞中rFSH刺激的孕激素产生的过程中起着至关重要的作用.
- 这一发现为在接受rFSH治疗的IVF患者中观察到的过早孕激素升高提供了潜在的分子机制.
更多相关视频
05:32Author Spotlight: Investigating the Relationship Between FSH and Pathophysiological Changes in Perimenopausal Women - Insights from a Mouse Model
Published on: August 11, 2023
1.6K
09:15Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Published on: May 23, 2014
22.7K
相关概念视频
Hormonal Regulation of the Menstrual Cycle
279
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
279
Ovarian Cycle
959
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
959
Hormonal Control of the Ovarian Cycle
396
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
396
Folliculogenesis
570
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
570
