循环ZmMED16通过与ZmAPS1的结合来负面调节粉含量,从而延迟了植物的开花
Xin Tang1,2, Xiaoju Feng1,2, Yang Xu1,2
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 610000, China.
Journal of integrative plant biology
|January 21, 2025
概括
在玉米中,一种新的圆形RNA,circZmMED16,通过与APS1mRNA相互作用来调节花期. 过度表达通过降低AGPase活性来延迟开花,从而揭示了植物中的新的circRNA功能.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 循环RNAs (circRNAs) 是植物基因表达的新兴调节者.
- 它们在植物发育和应激反应中的确切功能仍然在很大程度上是未知的.
- 了解circRNA的作用对于推动植物科学发展至关重要.
研究的目的:
- 识别和描述涉及植物发育的新型circRNAs.
- 为了阐明circZmMED16在玉米 (Zea mays L.) 开花时间中的特定功能.
- 揭示circZmMED16调节开花的分子机制.
主要方法:
- 用于识别的 circRNA 测序 (circRNA-seq).
- 在玉米和Arabidopsis thaliana中转基因表达.
- RNA下拉,双化酶试验,RNA干扰 (RNAi) 和核糖核酶保护试验 (RPA).
- 对ADP-葡萄糖铁酶 (AGPase) 活性进行分析.
主要成果:
- 确定了circZmMED16,在玉米中高度表达,在细胞质中起作用.
- 过度表达circZmMED16延迟了玉米和阿拉比多普西斯的开花.
- circZmMED16与ADP-葡萄糖热酶子单元1 (APS1) mRNA直接相互作用.
- 循环ZmMED16敲击增加了ZmAPS1表达;过度表达降低了ZmAPS1,降低了AGPase活性并延迟了开花.
结论:
- circZmMED16在调节开花时间方面发挥着特殊作用,与其线性对应物不同.
- circZmMED16通过与APS1mRNA相互作用,作为分子调节剂,影响AGPase活性.
- 这项研究揭示了一种新的circRNA介导的调节途径,可以控制植物的开花时间.
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