细胞ophidium复合体与细胞命运产生共
Yi-Lan Li1, Ji-Long Liu2,3
1School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Cellular and molecular life sciences : CMLS
|January 21, 2025
概括
代谢酶形成细胞结构称为cytoophidia. 它们组合成复合体与细胞状态相关,影响细胞命运和NAD生物合成.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 代谢过程中的代谢.
背景情况:
- 代谢对于生命至关重要.
- 2010年发现了cytoophidia,由CTP合成酶 (CTPS) 等代谢酶形成的类似蛇的结构.
- 不同细胞的分布和相互作用仍然不清楚.
研究的目的:
- 研究Saccharomyces cerevisiae中的五种代谢酶所形成的细胞的分布和关系:Asn1,Bna5,CTPS (Ura7),Glt1和Prs5.
- 了解细胞组合如何影响细胞命运和代谢途径.
主要方法:
- 在酵母细胞中观察细胞的形成和复杂的组合.
- 基因操纵以阻止cytoophidium的组装.
- 分析细胞表型 (静止与非静止) 和代谢途径活性 (NAD生物合成).
主要成果:
- 多个cytoophidia可以组装成更大的综合体.
- 在非静止细胞中,Glt1细胞是普遍存在的,而在静止细胞中,CTPS细胞更常见.
- 在CTPS中,cytoophidia与Prs5和Asn1.1形成复合体.
- 阻止CTPS细胞组合促进非静止状态,增加Glt1和Bna5细胞,并抑制NAD生物合成.
- 这种非静止的表型可以通过干预影响Glt1细胞,尼古丁酸补充或Sir2过度表达来挽救.
结论:
- 细胞ophidium复合体的组成与不同的细胞命运有关.
- 细胞的组合和相互作用在调节细胞静止和代谢状态,特别是NAD生物合成方面发挥着至关重要的作用.
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