自给自足的生物催化级联用于流动中danshensu的连续合成
Valentina Marchini1,2, Francesca Paradisi3
1Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.
Applied microbiology and biotechnology
|January 21, 2025
概括
一个新的双酶系统有效地使用固定酶产生danshensu用于辅因子再生. 这种可持续的方法在批量和连续流生物反应器中实现了高转换率和生产率.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 合成生物学 合成生物学
- 绿色化学 绿色化学
背景情况:
- 丹申苏是一种具有治疗潜力的活性成分.
- 需要有效和可持续的合成方法来生产danshensu.
- 基于酶的生物转化为传统化学合成提供了一个有希望的替代方案.
研究的目的:
- 开发一种新的双酶策略,用于高效的ex vivo生产danshensu.
- 为了描述来自Mentha x piperita的氧烯酸还原酶.
- 实施和优化用于danshensu合成的连续流生物反应器系统.
主要方法:
- 氨酸脱酶 (Bacillus sphaericus) 和基酸减少酶 (Mentha x piperita) 的联合固定.
- 使用固定酶与局部辅因子 (NAD+) 再生进行批量生物转化.
- 开发和运行一个用于连续流生物转化的包装式反应堆.
主要成果:
- 在24小时内在批量模式下实现了60%的L-多巴转化.
- 在连续流量中达到高达80%的转换,保持时间为60分钟.
- 证明了生物催化剂的稳定性,在48小时后保持62%的活性.
- 获得了1.84gL-1h-1的最终生产率,danshensu的纯度为96%.
结论:
- 开发的双酶系统为danshensu合成提供了可持续和高效的途径.
- 酶固定和连续流处理提高反应效率和催化剂稳定性.
- 该战略提供了一种绿色化学方法,用于生产有价值的生物活性化合物.
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