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通过自主进化突变检测高耐葡萄糖大肠杆菌的L-氨酸发酵

Li Zhang¹, Zhijie Cheng¹, Jing Jiang¹

¹College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 224051, PR China.

Applied microbiology and biotechnology

|January 21, 2025

概括

这项研究通过修改不匹配修复和突变发生基因,对大肠杆菌进行了L-氨酸生产的工程设计. 通过适应性进化和代谢工程,工程菌株通过适应性进化和代谢工程实现了6.62g/L的L-氨酸.

科学领域:

微生物学和代谢工程微生物学和代谢工程
合成生物学合成生物学

背景情况:

氨酸是一种重要的氨基酸,具有多种应用.
大肠杆菌是L-氨酸生产的关键工业微生物,由于其快速发酵和遗传可处理性.

研究的目的:

为了增强大肠杆菌中L-氨酸的产生.
通过有针对性的基因改造和适应性进化,开发出优越的微生物菌株.

主要方法:

对不匹配修复基因 (mutH,mutL,mutS,recG) 和突变基因 (dinB,rpoS,rpoD,recA) 的系统查.
利用高葡萄糖适应培养和代谢工程策略.
使用多片段无重组合组装L-氨酸生物合成途径基因 (ilvBN,ilvC,ilvE,ilvD).

主要成果:

工程化大肠杆菌从非生产者转变为熟练的L-氨酸生产者.
达到6.62g/L的最大L-氨酸产量.
在工程菌株中表现出增强的应力耐受性和L-氨酸积累.

结论:

自我进化和代谢工程的协同应用显著改善了L-瓦林的生产.
开发的大肠杆菌菌株为工业L-氨酸生物合成提供了一个有前途的平台.
针对特定基因的定向修改与适应性培养相结合,对菌株改善是有效的.

关键词:

美国大肠杆菌 (E. coli).葡萄糖耐受性是一种耐受性.这是一种L-氨酸.代谢修饰是一种代谢修饰.幸存率幸存率是指生存率.

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