IL-17家族成员在CF呼吸上皮细胞中施加自克林的亲炎循环ex vivo
Caterina Allegretta1, Enza Montemitro2, Fabiana Ciciriello2
1Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.
Cellular immunology
|January 21, 2025
概括
插白素-17 (IL-17) 细胞因子家族在呼吸系统细胞中得到广泛表达,并可能通过自身分泌循环放大囊性纤维化 (CF) 气道的炎症. 这项研究研究了CF细胞中的IL-17受体和细胞因子表达.
科学领域:
- 免疫学 免疫学 免疫学
- 肺部病理学 肺部病理学
- 细胞生物学 细胞生物学
背景情况:
- 囊性纤维化 (CF) 肺部表现出慢性炎症和 Pseudomonas aeruginosa 感染.
- 在CF唾液中发现了高水平的互白素-17A和F (IL-17A/F),这表明IL-17家族在CF肺部疾病中发挥了作用.
研究的目的:
- 研究从囊性纤维化患者培养的人类呼吸道上皮细胞中IL-17受体 (IL-17R) 和IL-17细胞因子的表达.
- 确定IL-17家族成员在CF气道内的炎症反应中的作用.
主要方法:
- 量化逆转录PCR (RT-qPCR) 用于测量CF支气管上皮细胞 (CFBE) 和CF初级鼻上皮细胞 (HNE) 中的IL-17受体和细胞因子的mRNA水平.
- 酶相关免疫吸收试验 (ELISA) 和Bioplex试验用于量化HNE细胞释放的细胞因子.
- 用Pseudomonas aeruginosa PAO1或从囊性纤维化患者中分离的外产品 (EXO) 刺激细胞.
主要成果:
- 感染PAO1或EXO显著上调CFBE细胞中的大多数IL-17RmRNA,但不包括IL-17RD.
- 对HNE细胞的EXO刺激增加了大多数IL-17细胞因子和受体 (不包括IL-17D/RD) 的mRNA水平,并诱导了IL-17A,IL-17B,IL-17C,IL-17E和IL-17F的释放.
- 暴露于IL-17A和IL-17F刺激了HNE细胞释放促炎细胞因子 (IL-1β,IL-6,TNF-α),中性细胞化学因子 (IL-8,G-CSF) 和参与粘素产生和离子运输的细胞因子 (IL-4,IL-13).
结论:
- IL-17家族在呼吸道上皮细胞中广泛表达,可能在CF气道中产生自身隐性炎症放大循环.
- 使用患者衍生细胞的体外研究强调了IL-17家族成员在囊性纤维化肺免疫反应中的重要参与.
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