在Arabidopsis中 PHO1;H1-介导的酸盐运输的基础结构机制
Sunzhenhe Fang1,2,3, Yang Yang1,2, Xue Zhang1,2
1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
Nature plants
|January 21, 2025
概括
阿拉比多普西斯的酸盐1 (AtPHO1;H1) 结构揭示了酸盐运输的新通道式机制. 这项研究阐明了SPX-EXS家族.
科学领域:
- 植物生物学 植物生物学
- 分子和结构生物学分子和结构生物学.
- 生物化学 生物化学
背景情况:
- 阿拉比多普西斯酸盐1 (AtPHO1) 和AtPHO1;H1是根到芽转移的关键酸盐运输体.
- 它们属于独特的SPX-EXS家族,结构和分子机制不明.
研究的目的:
- 确定与无机酸盐 (Pi) 和异醇六酸盐结合的AtPHO1;H1的结构.
- 阐明SPX-EXS家族中酸盐运输和调节的分子机制.
主要方法:
- 低温电子显微镜 (cryo-EM) 用于确定AtPHO1;H1.1.的结构.
- 模拟分子动力学和AlphaFold预测以探索形状变化.
- 结构和功能分析以了解基质结合和运输.
主要成果:
- 冷-EM结构显示了AtPHO1;H1在一个封闭的构造,与模拟支持一个开放的构造.
- 在PHO1;H1中形成一个域互换的同位体,涉及SPX和EXS域.
- 确定了一种具有独立基质路径和结合位点的新型EXS域折叠,由关门残留物Trp719和Tyr610调节.
- 在SPX域二元接口上结合伊诺西六基酸可增强AtPHO1;H1活性.
- 一个C端尾部对于AtPHO1;H1功能至关重要.
结论:
- 通过AtPHO1;H1和相关蛋白质调解的Pi流动的通道式机制被提出.
- 这项研究揭示了SPX-EXS家族Pi运输的分子和调节机制.
- 这些发现为工程作物提供了潜力,提高了用于可持续农业的利用效率.
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