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Jitu Wang^1,2, Na Liu^1,2, Mei Hu^1,2

¹Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

European journal of medical research

|January 22, 2025

概括

泌尿外体显示出特定的溶酶体相关蛋白质,CTSZ,AP1B1和LAMP2,作为监测急性痛风发作的潜在的非侵入性生物标志物. 这些发现为痛风患者提供了侵入性诊断方法的有希望的替代方案.

科学领域:

生物化学生化学
泌尿器科泌尿器科泌尿器科泌尿器科
生物标志物发现发现

背景情况:

自-溶解体通路与痛风的发展有关.
目前的痛风诊断和监测依赖于侵入性方法,缺乏对急性阶段的预测能力.
这项研究研究了尿液外体蛋白质的非侵入性痛风生物标志物.

研究的目的:

识别和评估尿道外体中的溶酶体相关蛋白质,作为急性痛风发作的潜在生物标志物.
探索痛风的非侵入性诊断和监测策略.

主要方法:

从急性痛风 (AD),间歇性痛风 (ID) 和正常对照 (NC) 组收集尿液样本.
分离出尿外体,并使用DIA质谱分析蛋白质表达.
对于差异表达的蛋白质,生成了接收器操作特征 (ROC) 曲线.

主要成果:

在1896个检测到的蛋白质中,在AD和ID组之间确定了121种不同表达的蛋白质.
三种与溶酶体相关的蛋白质,Cathepsin Z (CTSZ),AP-1复合体子单元β-1 (AP1B1) 和溶酶体相关的膜糖蛋白2 (LAMP2),显示出显著的差异表达.
在监测急性痛风时,ROC分析显示了CTSZ (AUC=0.826),AP1B1 (AUC=0.847) 和LAMP2 (AUC=0.882) 的强有力的预测值.

结论:

尿道外体内含有特定的蛋白质变化,患者经历急性痛风发作.
尿道外体体中的酶相关蛋白CTSZ,AP1B1和LAMP2显示出作为监测急性痛风的新生物标志物的潜力.
这些生物标志物可能为痛风管理提供一种非侵入性的方法.

关键词:

发生急性痛风发作.自自是一种自的过程.生物标志物生物标志物 lysosome 是一个溶解体.尿液外生体是尿液中的外生体.

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