相关实验视频
Updated: Jun 1, 2025
08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
7.8K
LINC00941通过调节miR-335-5p/KAT7轴影响骨质细胞的增殖,亡和分化
Longjin Liu1, Ye Yang2, Pengxiao Sun3
1Department of Orthopedic 2, Zhongxian People's Hospital of Chongqing, Chongqing, 404300, China.
Journal of orthopaedic surgery and research
|January 22, 2025
概括
长非编码RNA LINC00941 在延迟骨折愈合 (DFH) 中被上调. 它通过miR-335-5p/KAT7通路抑制骨质细胞功能,这表明DFH的治疗标.
科学领域:
- 整形外科和再生医学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 骨折是常见的骨科损伤,大约10%的骨折经历了延迟的骨折愈合 (DFH).
- 了解DFH背后的分子机制对于开发有效的治疗方法至关重要.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在细胞过程中的作用,包括骨愈合.
研究的目的:
- 调查LINC00941在延迟骨折愈合 (DFH) 中的作用.
- 阐明LINC00941在调节骨质细胞分化的基础分子机制.
- 探索miR-335-5p/KAT7轴在LINC00941-介导的DFH中的参与.
主要方法:
- 定量实时PCR (RT-qPCR) 用于评估基因表达水平.
- 在MC3T3-E1细胞中进行细胞增殖试验 (CCK-8) 和亡分析 (流细胞计).
- 双露西法酶记者基因测定证实了LINC00941,miR-335-5p和KAT7.7之间的调控关系.
主要成果:
- 在DFH患者中,LINC00941表达显著上调,而miR-335-5p则下调.
- LINC00941被确定为DFH的潜在诊断标记物.
- LINC00941抑制了骨质细胞的增殖和分化,同时促进了通过miR-335-5p/KAT7轴介导的亡.
结论:
- LINC00941/miR-335-5p/KAT7轴在延迟骨折愈合的发病过程中发挥着至关重要的作用.
- 准这一轴为治疗DFH提供了一个潜在的治疗策略.
相关概念视频
01:31Osteoclasts in Bone Remodeling
2.8K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.8K
01:16TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
01:40Bone Remodeling
38.1K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.1K
01:22PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K