作为食品加工酶固定化载体的一种新奇的奇托桑微球及其在核酸生产中的应用
Xiao-Yan Yin1, Rui-Fan Yang2, Zhong-Hua Yang1
1Xingzhi College, Zhejiang Normal University, Jinhua 321100, China.
Food chemistry: X
|January 22, 2025
概括
新型交联素微球 (CSM) 有效地固定了核酶P1 (NP1),提高了酶稳定性和可用于食品加工应用中的核酸生产的可重复使用性.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 食品科学 食品科学 食品科学
背景情况:
- 酶固定是工业应用的关键,需要稳定和安全的载体.
- 基托微球 (CSM) 提供了作为固定矩阵的潜力.
- 核酶P1 (NP1) 对于核酸生产有价值,但需要稳定.
研究的目的:
- 开发用于酶固定化的新型交联酸盐微球 (CSM).
- 将核酶P1 (NP1) 固定在已开发的CSM上 (NP1@CSMs-GA).
- 为了评估RNA水解的固定NP1的稳定性,活性和可重复使用性.
主要方法:
- 使用谷氨 (GA) 通过乳化-中和方法制备交联素微球 (CSM).
- 核酶P1 (NP1) 在CSMs上的固定.
- 对NP1@CSMs-GA活动,稳定性和运动参数 (Vmax,Km) 的表征.
- 在RNA水解中的NP1@CSMs-GA的应用,用于批量式坦克反应堆 (BSTR) 和包装床反应堆 (PBR) 中的核酸生产.
主要成果:
- 最大NP1@CSMs-GA活动达到53,859.4U/g,活动恢复收益率为75%.
- 与自由NP1.1相比,稳定性显著提高和动力参数发生变化 (Vmax = 895.71毫克/g·min),Km = 77.27毫克/毫升).
- 在BSTR中,NP1@CSMs-GA在10个重复使用周期后保留了超过75.1%的初始活动.
- 在PBR中24小时连续运行后,保持了81%的RNA水解转化率.
结论:
- 开发的交联酸盐微球为核P1固定提供了强大而安全的载体.
- NP1@CSMs-GA表现出卓越的稳定性和可重复使用性,使其适合工业核酸生产.
- 这种固定化的酶系统在RNA水解的批量和连续反应堆配置中表现出色.
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