骨质细胞可视化:使用NewFuchsin与非水性安装和组织清除兼容的耐酸酸酶活性染色
Takashi Nakamura1,2, Katsuhiro Kawaai3, Yukiko Kuroda3
1Department of Biochemistry, Tokyo Dental College, 2-9-18 Kanda-Misakicho, Chiyoda-ku, Tokyo 101-0061, Japan.
MethodsX
|January 22, 2025
概括
一种用于耐酸酸酶 (TRAP) 染色的新方法使用NewFuchsin,改善骨质组织学中的骨质细胞可视化. 这种技术允许长期保存和3D成像,克服传统TRAP染色的局限性.
科学领域:
- 历史学 历史学 历史学
- 生物化学 生化学
- 细胞生物学 细胞生物学
背景情况:
- 耐酸酸酶 (TRAP) 染色对于识别骨骨质细胞至关重要.
- 传统的TRAP染色方法产生在有机溶剂中溶解的染料,阻碍了样品的长期保存.
- 与传统的TRAP染色使用的水性安装介质也限制了样品存储时间.
研究的目的:
- 开发一种修改后的TRAP染色方法,以提高组织学图像质量和长期保存.
- 为了在明亮场和光显微镜中检测TRAP活动.
- 为了促进骨质细胞的三维成像.
主要方法:
- 一个修改的TRAP染色协议,使用NewFuchsin作为染色基因.
- 在染色后应用脱水和清除步骤.
- 使用非水性有机溶剂基的安装介质.
- 与乙基酸盐集成用于组织清除和3D成像.
主要成果:
- 新Fuchsin TRAP染色产生稳定的红色染料,允许脱水,清除和使用有机装配介质.
- 染色部分呈现出清晰的组织学图像,适合长期保存.
- 在明亮场和光显微镜中都可以检测到TRAP活动.
- 结合组织清除,骨质细胞可以使用三维成像来可视化.
结论:
- 新富辛的TRAP染色方法比传统的骨质细胞识别技术具有显著的优势.
- 这种修改后的方法提高了样本的保存,并扩大了成像的可能性,包括光和3D重建.
- 新Fuchsin TRAP染色是骨科研究的一个有价值的工具,提供了更清晰,更持久的组织学分析.
关键词:
在 Acp5p5 中使用.骨头 骨头 骨头 骨头骨头的切口是骨头的切口.脱水 脱水 脱水 脱水 脱水装载媒体的安装媒体.新福克斯在TRAP上的染色非水性安装非水性安装有机溶剂有机溶剂骨质细胞染色的染色这就是TRAP TRAP.组织清除 组织清除更多相关视频
12:55Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
Published on: October 10, 2017
8.9K
05:23Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
Published on: May 20, 2022
2.6K
相关概念视频
01:03Fixation and Sectioning
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
01:24Simple Staining Technique
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
01:26Differential Staining Technique
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
01:13Special Staining Techniques
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...