Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:24

DNA Isolation

37.9K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
37.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Thiol-Redox Biochemistry of Trypanosomatids: A New Perspective Based on Known and New Actors.

Biochemistry·2026
Same author

MSRB3 antioxidant activity is necessary for inner ear cuticular plate structure and hair bundle integrity.

Disease models & mechanisms·2025
Same author

Revisiting the y-ome of Escherichia coli.

Nucleic acids research·2024
Same author

SAS: Split antibiotic selection for identifying chaperones that improve protein solubility.

Heliyon·2024
Same author

Catalytic Mechanism of <i>Mycobacterium tuberculosis</i> Methionine Sulfoxide Reductase A.

Biochemistry·2024
Same author

Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli.

Nature communications·2023

相关实验视频

Updated: May 31, 2025

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
10:32

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Published on: December 6, 2017

11.1K

从基因组DNA构建等离子体图书馆

Valeria Florez-Cardona1,2, Jessica Khani1, Emily McNutt1

  • 1New England Biolabs, Ipswich, Massachusetts.

Current protocols
|January 22, 2025
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种简化,可扩展的方法来构建基因组DNA等离子体库,更新了一个40年前的协议. 新的程序简化了使用功能基因组学和下一代测序 (NGS) 的基因功能发现.

关键词:
细菌选择 细菌选择基因组 DNA DNA 的基因组.塑图书馆建设 塑图书馆建设

更多相关视频

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.3K
Large Insert Environmental Genomic Library Production
20:59

Large Insert Environmental Genomic Library Production

Published on: September 23, 2009

15.9K

相关实验视频

Last Updated: May 31, 2025

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
10:32

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Published on: December 6, 2017

11.1K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.3K
Large Insert Environmental Genomic Library Production
20:59

Large Insert Environmental Genomic Library Production

Published on: September 23, 2009

15.9K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 功能性基因组方法对于识别基因功能和将它们与表型联系起来至关重要.
  • 传统的基因组DNA等离子体图书馆建设协议在40年来几乎没有创新.
  • 现有的方法往往复杂且耗时,阻碍了快速的基因发现.

研究的目的:

  • 介绍一种新的,简化的,可扩展的程序,用于从基因组DNA构建等离子体库.
  • 提供一个更新的协议,克服功能基因组学现有方法的局限性.
  • 通过概念验证图书馆构建和测序来证明新协议的有效性.

主要方法:

  • 使用g-TUBE进行基因组DNA提取和物理碎片化.
  • 使用磁珠修复和选择性净化2.5kb的碎片.
  • 结合到一个粗末消化的载体,然后在高效的大肠杆菌和等离子体DNA提取中进行放大.

主要成果:

  • 从不同的基因组中成功构建和测序了三个基因组库.
  • 使用下一代测序 (NGS) 工作流程计算图书馆覆盖范围.
  • 新的程序是可扩展的,使用常见的实验室试剂,并依赖于简单的技术.

结论:

  • 开发的协议为构建基因组DNA等离子体库提供了显著的改进.
  • 这种最新的方法促进了功能性基因组研究和基因发现.
  • 该协议强大,高效,适合各种基因组应用.